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New generation of efficient peptide-based vectors, NickFects, for the delivery of nucleic acids
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2013 (English)In: Biochimica et Biophysica Acta - Biomembranes, ISSN 0005-2736, E-ISSN 1879-2642, Vol. 1828, no 5, 1365-1373 p.Article in journal (Refereed) Published
Abstract [en]

Harnessing of a branched structure is a novel approach in the design of cell-penetrating peptides and it has provided highly efficient transfection reagents for intracellular delivery of nucleic acids. The new stearylated TP10 analogs, NickFects, condense plasmid DNA, splice correcting oligonucleotides and short interfering RNAs into stable nanoparticles with a size of 62-160 nm. Such nanoparticles have a negative surface charge (-11 to -18 mV) in serum containing medium and enable highly efficient gene expression, splice correction and gene silencing. One of the novel peptides, NickFect51 is capable of transfecting plasmid DNA into a large variety of cell lines, including refractory suspension and primary cells and in several cases exceeds the transfection level of commercially available reagent Lipofectamine (TM) 2000 without any cytotoxic side effects. Additionally we demonstrate the advantages of NickFect51 in a protein production system, QMCF technology, for expression and production of recombinant proteins in hardly transfectable suspension cells.

Place, publisher, year, edition, pages
2013. Vol. 1828, no 5, 1365-1373 p.
Keyword [en]
Plasmid transfection, Cell-penetrating peptide, Splice-correction, Gene silencing, Protein production, QMCF technology
National Category
Biochemistry and Molecular Biology Biophysics
Identifiers
URN: urn:nbn:se:su:diva-89854DOI: 10.1016/j.bbamem.2013.01.011ISI: 000317165800005OAI: oai:DiVA.org:su-89854DiVA: diva2:621443
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AuthorCount:14;

Available from: 2013-05-14 Created: 2013-05-14 Last updated: 2017-12-06Bibliographically approved

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Langel, Ülo
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Department of Neurochemistry
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