Conformational Preferences of the O-Antigen Polysaccharides of Escherichia coli O5ac and O5ab Using NMR Spectroscopy and Molecular Modeling
2013 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 14, no 7, 2215-2224 p.Article in journal (Refereed) Published
Escherichia coli serogroup O5 comprises two different subgroups (O5ab and O5ac), which are indiscernible from the point of view of standard immunological serotyping. The structural similarities between the O-antigen polysaccharides (PSs) of these two strains are remarkable, with the only difference being the glycosidic linkage connecting the biological tetrasaccharide repeating units. In the present study, a combination of NMR spectroscopy and molecular modeling methods were used to elucidate the conformational preferences of these two PSs. The NMR study was based on the analysis of intra- and inter-residue proton-proton distances using NOE build-up curves. Molecular models of the repeating units and their extension to polysaccharides were obtained, taking into account the conformational flexibility as assessed by the force field applied and a genetic algorithm. The agreements between experimentally measured and calculated distances could only be obtained by considering an averaging of several low energy conformations observed in the molecular models.
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2013. Vol. 14, no 7, 2215-2224 p.
Research subject Organic Chemistry
IdentifiersURN: urn:nbn:se:su:diva-92630DOI: 10.1021/bm400354yISI: 000321793700011OAI: oai:DiVA.org:su-92630DiVA: diva2:641748
FunderSwedish Research CouncilKnut and Alice Wallenberg FoundationEU, FP7, Seventh Framework Programme