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The actinorhizal symbiosis of Datisca glomerata: Search for nodule-specific marker genes
Stockholm University, Faculty of Science, Department of Ecology, Environment and Plant Sciences.
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The actinorhizal symbiosis is entered by nitrogen-fixing actinobacteria of the genus Frankia and a large group of woody plant species distributed among eight dicot families. The actinorhizal symbiosis, as well as the legume-rhizobia symbiosis, involves the stable intracellular accommodation of the microsymbionts in special organs called root nodules. Within the nodules, the nitrogen-fixing bacteria are provided with carbon sources by the host plant while supplying the plant with fixed nitrogen, which is often a limiting factor in plant growth and development.

Datisca glomerata (C. Presl.) Baill. (Datiscaceae, Cucurbitales) is a suffruticose plant with a relatively short generation time of six months, and therefore represents the actinorhizal species most suited as a genetic model system. In order to obtain an overview of nodule development and metabolism, the nodule transcriptome was analyzed. Comparison of nodule vs. root transcriptomes allowed identification of potential marker genes for nodule development. The activity of the promoters of two of these genes was studied in planta. Furthermore, auxins and cytokinins were quantified in roots and nodules, and the auxin responses in roots were compared in D. glomerata and the model legume Medicago truncatula.

Our results indicate that in actinorhizal plants signaling in the root epidermis leading to nodule organogenesis follows the common symbiosis pathway described for the legume-rhizobia symbiosis and arbuscular mycorrhiza. Moreover, we discovered a group of nodule-specific genes encoding defensin-like peptides with an unusual C-terminal domain that had not been found in other plant species. A possible role in the symbiosis-specific differentiation of the microsymbiont and in nodule development was suggested for these cysteine-rich peptides. Finally, we showed that D. glomerata and M. truncatula differ in their auxin and cytokinin requirements for the development of both nodules and lateral roots.

Place, publisher, year, edition, pages
Stockholm: Department of Ecology, Environment and Plant Sciences, Stockholm University , 2013. , 46 p.
Keyword [en]
actinorhizal symbiosis, Datisca glomerata, nodule transcriptome, nodule-specific marker genes, cysteine-rich peptides, auxins and cytokinins
National Category
Botany
Research subject
Plant Physiology
Identifiers
URN: urn:nbn:se:su:diva-92857ISBN: 978-91-7447-691-0 (print)OAI: oai:DiVA.org:su-92857DiVA: diva2:642865
Public defence
2013-09-25, föreläsningssalen, Institutionen för ekologi, miljö och botanik, Lilla Frescativägen 5, Stockholm, 13:00 (English)
Opponent
Supervisors
Note

At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 1: In press. Paper 2: Manuscript. Paper 3: Manuscript. Paper 4: Manuscript.

Available from: 2013-09-03 Created: 2013-08-22 Last updated: 2013-10-01Bibliographically approved
List of papers
1. Comparison of the Nodule vs. Root Transcriptome of the Actinorhizal Plant Datisca glomerata: Actinorhizal Nodules Contain a Specific Class of Defensins
Open this publication in new window or tab >>Comparison of the Nodule vs. Root Transcriptome of the Actinorhizal Plant Datisca glomerata: Actinorhizal Nodules Contain a Specific Class of Defensins
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2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 8, e72442- p.Article in journal (Refereed) Published
Abstract [en]

Actinorhizal root nodule symbioses are very diverse, and the symbiosis of Datisca glomerata has previously been shown to have many unusual aspects. In order to gain molecular information on the infection mechanism, nodule development and nodule metabolism, we compared the transcriptomes of D. glomerata roots and nodules. Root and nodule libraries representing the 3'-ends of cDNAs were subjected to high-throughput parallel 454 sequencing. To identify the corresponding genes and to improve the assembly, Illumina sequencing of the nodule transcriptome was performed as well. The evaluation revealed 406 differentially regulated genes, 295 of which (72.7%) could be assigned a function based on homology. Analysis of the nodule transcriptome showed that genes encoding components of the common symbiosis signaling pathway were present in nodules of D. glomerata, which in combination with the previously established function of SymRK in D. glomerata nodulation suggests that this pathway is also active in actinorhizal Cucurbitales. Furthermore, comparison of the D. glomerata nodule transcriptome with nodule transcriptomes from actinorhizal Fagales revealed a new subgroup of nodule-specific defensins that might play a role specific to actinorhizal symbioses. The D. glomerata members of this defensin subgroup contain an acidic C-terminal domain that was never found in plant defensins before.

National Category
Natural Sciences
Identifiers
urn:nbn:se:su:diva-94186 (URN)10.1371/journal.pone.0072442 (DOI)000323734600020 ()
Note

AuthorCount:5;

Fundin Agency:

Forskningsradet Formas (Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning)  2005-2773-3234-80;  Vetenskapsradet (Swedish Research Council)  2007-17840-52674-16;  Fundacao para a Ciencia e Tecnologia (FCT)  SFRH/BPD/43228/2008

Available from: 2013-10-01 Created: 2013-09-30 Last updated: 2017-12-06Bibliographically approved
2. Cysteine-rich peptide genes are expressed in the infected nodule cells of the actinorhizal plant Datisca glomerata
Open this publication in new window or tab >>Cysteine-rich peptide genes are expressed in the infected nodule cells of the actinorhizal plant Datisca glomerata
(English)Manuscript (preprint) (Other academic)
National Category
Botany
Research subject
Plant Physiology
Identifiers
urn:nbn:se:su:diva-92989 (URN)
Available from: 2013-08-28 Created: 2013-08-28 Last updated: 2013-08-28Bibliographically approved
3. Candidatus Frankia datiscae Dg1, the actinobacterial symbiont of Datisca glomerata, is a member of the basal clade of symbiotic frankiae and expresses the common nod genes
Open this publication in new window or tab >>Candidatus Frankia datiscae Dg1, the actinobacterial symbiont of Datisca glomerata, is a member of the basal clade of symbiotic frankiae and expresses the common nod genes
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(English)Manuscript (preprint) (Other academic)
National Category
Botany
Identifiers
urn:nbn:se:su:diva-92990 (URN)
Available from: 2013-08-28 Created: 2013-08-28 Last updated: 2013-08-28Bibliographically approved
4. Auxins and cytokinins in roots, hairy roots and nodules of the actinorhizal plant Datisca glomerata and the model legume Medicago truncatula
Open this publication in new window or tab >>Auxins and cytokinins in roots, hairy roots and nodules of the actinorhizal plant Datisca glomerata and the model legume Medicago truncatula
Show others...
(English)Manuscript (preprint) (Other academic)
National Category
Botany
Identifiers
urn:nbn:se:su:diva-92991 (URN)
Available from: 2013-08-28 Created: 2013-08-28 Last updated: 2013-08-28Bibliographically approved

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