Dynamics of exocyclic groups in the Escherichia coli O91 O-antigen polysaccharide in solution studied by carbon-13 NMR relaxation
2013 (English)In: Journal of Biomolecular NMR, ISSN 0925-2738, E-ISSN 1573-5001, Vol. 57, no 1, 37-45 p.Article in journal (Refereed) Published
Carbon-13 relaxation data are reported for exocyclic groups of hexopyranosyl sugar residues in the repeating unit within the Escherichia coli O91 O-antigen polysaccharide in a dilute D2O solution. The measurements of T 1, T 2 and heteronuclear nuclear Overhauser enhancements were carried out at 310 K at two magnetic fields (16.4 T, 21.1 T). The data were analyzed using the standard and extended Lipari–Szabo models, as well as a conformational jump model. The extended version of the Lipari–Szabo and the two-site jump models were most successful for the hydroxymethyl groups of Gal and GlcNAc sugar residues. Different dynamics was found for the hydroxymethyl groups associated with different configurations (d-gluco, d-galacto) of the sugar residues, the latter being faster than the former.
Place, publisher, year, edition, pages
Springer Netherlands, 2013. Vol. 57, no 1, 37-45 p.
Carbohydrates, Polysaccharide, Biopolymer, NMR spectroscopy, Model free approach, Two-site jump model
Research subject Physical Chemistry
IdentifiersURN: urn:nbn:se:su:diva-92898DOI: 10.1007/s10858-013-9763-5ISI: 000323673800005OAI: oai:DiVA.org:su-92898DiVA: diva2:643113
FunderSwedish Research Council, 613-2011-3311Swedish Research Council, 621-2010-4960Knut and Alice Wallenberg Foundation