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Cysteine-rich peptide genes are expressed in the infected nodule cells of the actinorhizal plant Datisca glomerata
Stockholm University, Faculty of Science, Department of Ecology, Environment and Plant Sciences.
Stockholm University, Faculty of Science, Department of Ecology, Environment and Plant Sciences.
(English)Manuscript (preprint) (Other academic)
National Category
Botany
Research subject
Plant Physiology
Identifiers
URN: urn:nbn:se:su:diva-92989OAI: oai:DiVA.org:su-92989DiVA: diva2:643613
Available from: 2013-08-28 Created: 2013-08-28 Last updated: 2013-08-28Bibliographically approved
In thesis
1. The actinorhizal symbiosis of Datisca glomerata: Search for nodule-specific marker genes
Open this publication in new window or tab >>The actinorhizal symbiosis of Datisca glomerata: Search for nodule-specific marker genes
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The actinorhizal symbiosis is entered by nitrogen-fixing actinobacteria of the genus Frankia and a large group of woody plant species distributed among eight dicot families. The actinorhizal symbiosis, as well as the legume-rhizobia symbiosis, involves the stable intracellular accommodation of the microsymbionts in special organs called root nodules. Within the nodules, the nitrogen-fixing bacteria are provided with carbon sources by the host plant while supplying the plant with fixed nitrogen, which is often a limiting factor in plant growth and development.

Datisca glomerata (C. Presl.) Baill. (Datiscaceae, Cucurbitales) is a suffruticose plant with a relatively short generation time of six months, and therefore represents the actinorhizal species most suited as a genetic model system. In order to obtain an overview of nodule development and metabolism, the nodule transcriptome was analyzed. Comparison of nodule vs. root transcriptomes allowed identification of potential marker genes for nodule development. The activity of the promoters of two of these genes was studied in planta. Furthermore, auxins and cytokinins were quantified in roots and nodules, and the auxin responses in roots were compared in D. glomerata and the model legume Medicago truncatula.

Our results indicate that in actinorhizal plants signaling in the root epidermis leading to nodule organogenesis follows the common symbiosis pathway described for the legume-rhizobia symbiosis and arbuscular mycorrhiza. Moreover, we discovered a group of nodule-specific genes encoding defensin-like peptides with an unusual C-terminal domain that had not been found in other plant species. A possible role in the symbiosis-specific differentiation of the microsymbiont and in nodule development was suggested for these cysteine-rich peptides. Finally, we showed that D. glomerata and M. truncatula differ in their auxin and cytokinin requirements for the development of both nodules and lateral roots.

Place, publisher, year, edition, pages
Stockholm: Department of Ecology, Environment and Plant Sciences, Stockholm University, 2013. 46 p.
Keyword
actinorhizal symbiosis, Datisca glomerata, nodule transcriptome, nodule-specific marker genes, cysteine-rich peptides, auxins and cytokinins
National Category
Botany
Research subject
Plant Physiology
Identifiers
urn:nbn:se:su:diva-92857 (URN)978-91-7447-691-0 (ISBN)
Public defence
2013-09-25, föreläsningssalen, Institutionen för ekologi, miljö och botanik, Lilla Frescativägen 5, Stockholm, 13:00 (English)
Opponent
Supervisors
Note

At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 1: In press. Paper 2: Manuscript. Paper 3: Manuscript. Paper 4: Manuscript.

Available from: 2013-09-03 Created: 2013-08-22 Last updated: 2013-10-01Bibliographically approved

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Demina, Irina V.Pawlowski, Katharina
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