Improved production of membrane proteins in Escherichia coli by selective codon substitutions
2013 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 587, no 15, 2352-2358 p.Article in journal (Refereed) Published
Membrane proteins are extremely challenging to produce in sufficient quantities for biochemical and structural analysis and there is a growing demand for solutions to this problem. In this study we attempted to improve expression of two difficult-to-express coding sequences (araH and narK) for membrane transporters. For both coding sequences, synonymous codon substitutions in the region adjacent to the AUG start led to significant improvements in expression, whereas multi-parameter sequence optimization of codons throughout the coding sequence failed. We conclude that coding sequences can be re-wired for high-level protein expression by selective engineering of the 5' coding sequence with synonymous codons, thus circumventing the need to consider whole sequence optimization. (C) 2013 Federation of European Biochemical Societies.
Place, publisher, year, edition, pages
2013. Vol. 587, no 15, 2352-2358 p.
Membrane protein, Transporter, Over-expression, Synthetic coding sequence, Codon optimization, AraH, NarK, Escherichia coli
Biochemistry and Molecular Biology
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:su:diva-93298DOI: 10.1016/j.febslet.2013.05.063ISI: 000322606000010OAI: oai:DiVA.org:su-93298DiVA: diva2:646123
FunderSwedish Research Council