Structural studies of the O-antigen polysaccharide from Escherichia coli O115 and biosynthetic aspects thereof
2013 (English)In: Glycobiology, ISSN 0959-6658, E-ISSN 1460-2423, Vol. 23, no 3, 354-362 p.Article in journal (Refereed) Published
The structure of the O-antigen polysaccharide (PS) of Escherichia coliO115 has been investigated using a combination of component analysis and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy experiments. The repeating unit of the O-antigen was elucidated using the O-deacetylated PS and has the following branched pentasaccharide structure: →3)[β-L-Rhap-(1 → 4)]-β-D-GlcpNAc-(1 → 4)-α-D-GalpA-(1 → 3)-α-D-Manp-(1 → 3)-β-D-GlcpNAc-(1→. Cross-peaks of low intensity, corresponding to a β-L-Rhap-(1 → 4)-β-D-GlcpNAc-(1→ structural element, were present in the NMR spectra and attributed to the terminal part of the PS; this information defines the biological repeating unit of the O-antigen by having a 3-substituted N-acetyl-D-glucosamine (GlcNAc) residue at its reducing end. Analysis of the NMR spectra of the native PS revealed O-acetyl groups distributed over different positions of theL-Rhap residue (∼0.70 per repeating unit) as well as at O-2 and O-3 of the D-GalpA residue (∼0.03 and ∼0.25 per repeating unit, respectively), which is in agreement with the presence of two acetyltransferases previously identified in the O-antigen gene cluster (Wang Q, Ruan X, Wei D, Hu Z, Wu L, Yu T, Feng L, Wang L. 2010. Mol Cell Probes. 24:286–290.). In addition, the four glycosyltransferases initially identified in the O-antigen gene cluster of E. coli O115 were analyzed using BLAST, and the function of two of them predicted on the basis of similarities with glycosyltransferases from Shigella dysenteriae type 5 and 12, as well as E. coli O58 and O152.
Place, publisher, year, edition, pages
2013. Vol. 23, no 3, 354-362 p.
Escherichia coli, glycosyltransferases, lipopolysaccharide, O-acetylation, O-antigen
Research subject Organic Chemistry
IdentifiersURN: urn:nbn:se:su:diva-93837DOI: 10.1093/glycob/cws161OAI: oai:DiVA.org:su-93837DiVA: diva2:649194
FunderEU, FP7, Seventh Framework Programme, 215536Swedish Research CouncilKnut and Alice Wallenberg Foundation