In situ sequencing for RNA analysis in preserved tissue and cells
2013 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 10, no 9, 857-+ p.Article in journal (Refereed) Published
Tissue gene expression profiling is performed on homogenates or on populations of isolated single cells to resolve molecular states of different cell types. In both approaches, histological context is lost. We have developed an in situ sequencing method for parallel targeted analysis of short RNA fragments in morphologically preserved cells and tissue. We demonstrate in situ sequencing of point mutations and multiplexed gene expression profiling in human breast cancer tissue sections.
Place, publisher, year, edition, pages
2013. Vol. 10, no 9, 857-+ p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-94029DOI: 10.1038/nmeth.2563ISI: 000323760000020OAI: oai:DiVA.org:su-94029DiVA: diva2:651781