Change search
ReferencesLink to record
Permanent link

Direct link
Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
Show others and affiliations
2013 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 8, no 7, e69813- p.Article in journal (Refereed) Published
Abstract [en]

Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed a digital proximity ligation assay for protein measurement in fluids based on amplified single molecule detection. The assay showed significant improvements in precision, and thereby also detection sensitivity, over the conventional real-time PCR readout.

Place, publisher, year, edition, pages
2013. Vol. 8, no 7, e69813- p.
National Category
Natural Sciences
URN: urn:nbn:se:su:diva-94590DOI: 10.1371/journal.pone.0069813ISI: 000322064300117OAI: diva2:654023
Knut and Alice Wallenberg FoundationSwedish Research Council


Available from: 2013-10-07 Created: 2013-10-07 Last updated: 2013-10-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Nilsson, Mats
By organisation
Department of Biochemistry and BiophysicsScience for Life Laboratory (SciLifeLab)
In the same journal
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 20 hits
ReferencesLink to record
Permanent link

Direct link