Low doses of γ-radiation induce consistent protein expression changes in human leukocytes
2011 (English)In: International Journal of Low Radiation, ISSN 1477-6545, Vol. 8, no 5/6, 374-387 p.Article in journal (Refereed) Published
Twenty percent of cancer patients experience adverse effects after radiotherapy. The therapeutic doses are adjusted to the most sensitive individuals, resulting in a suboptimal dose for many patients. At present there is no screening system available to predict individual radiosensitivity. The main aim of this study is to investigate differences in protein expression pathways induced by low doses of radiation in whole blood obtained from radiosensitive and non–radiosensitive patients. To address this aim, a protocol for exposure condition, dose and analysis of whole blood was developed. The conditions for handling blood samples were optimised. The optimal doses and post irradiation conditions were determined. We found that 1 mGy and 150 mGy significantly changed protein expression in leukocytes collected from the two donors. The result shows that the protocol developed is suitable for characterisation of proteomic profiles associated with low dose exposure of leukocytes.
Place, publisher, year, edition, pages
Inderscience Publishers, 2011. Vol. 8, no 5/6, 374-387 p.
low dose radiation; Oxidative stress; proteomics; whole blood; gamma radiation
Cell Biology Other Biological Topics
Research subject Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-97111DOI: 10.1504/IJLR.2011.047188OAI: oai:DiVA.org:su-97111DiVA: diva2:675479
FunderSwedish Radiation Safety AuthoritySwedish Cancer Society