Change search
ReferencesLink to record
Permanent link

Direct link
Element analysis of the eutardigrades Richtersius coronifer and Milnesium cf. asiaticum using particle induced X-ray emission (PIXE)
Stockholm University, Faculty of Science, Department of Genetics, Microbiology and Toxicology. Kristianstad University .
2013 (English)In: Journal of limnology, ISSN 1129-5767, E-ISSN 1723-8633, Vol. 72, 92-101 p.Article in journal (Refereed) Published
Abstract [en]

Semi-terrestrial tardigrades are well-known for their tolerance to a variety of environmental extremes, including desiccation, freezing and radiation. Despite several attempts to reveal the genetic and molecular mechanisms behind the resilience of tardigrades, it is still unknown how these animals are able to maintain the integrity of their cellular components under severe stress. Quantitative or qualitative changes in molecular compounds (e.g., carbohydrates, proteins) are expected, and have been the main line of research towards understanding the tolerance of tardigrades. In radiation tolerant bacteria, a tolerance mechanism based on manganese has been proposed. We evaluate this hypothesis in tardigrades and provide the first data on element composition in desiccated and non-desiccated specimens of two eutardigrade species, Richtersius coronifer and Milnesium cf. asiaticum. A focused 2 MeV proton microbeam was utilised to determine the elemental content, distributions and concentrations, using the ion beam analytical technique particle induced X-ray emission (PIXE). The presence of six elements - phosphorus, sulphur, chlorine, potassium, calcium and iron - were confirmed in all tardigrade specimens, at levels up to a few mg g(-1). However, manganese was found in less than 10% of the analysed specimens, and in low amounts, thus our study provides no evidence for the manganese hypothesis. We also show that the distributions and/or concentrations of some elements differ between the two species as well as between the dehydrated and hydrated state. In particular, very low levels of iron were found in dehydrated M. cf. asiaticum. Our analysis shows that the PIXE technique is a useful tool for investigating questions on the distribution of elements both in dehydrated and hydrated tardigrades.

Place, publisher, year, edition, pages
2013. Vol. 72, 92-101 p.
Keyword [en]
tardigrades, Richtersius coronifer, Milnesium cf. asiaticum, PIXE, radiation tolerance
National Category
Natural Sciences
URN: urn:nbn:se:su:diva-98101DOI: 10.4081/jlimnol.2013.s1.e11ISI: 000327485200011OAI: diva2:682302


Available from: 2013-12-27 Created: 2013-12-27 Last updated: 2013-12-27Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Department of Genetics, Microbiology and Toxicology
In the same journal
Journal of limnology
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 81 hits
ReferencesLink to record
Permanent link

Direct link