Protein Expansion Is Primarily due to Indels in Intrinsically Disordered Regions
2013 (English)In: Molecular biology and evolution, ISSN 0737-4038, E-ISSN 1537-1719, Vol. 30, no 12, 2645-2653 p.Article in journal (Refereed) Published
Proteins evolve not only through point mutations but also by insertion and deletion events, which affect the length of the protein. It is well known that such indel events most frequently occur in surface-exposed loops. However, detailed analysis of indel events in distantly related and fast-evolving proteins is hampered by the difficulty involved in correctly aligning such sequences. Here, we circumvent this problem by first only analyzing homologous proteins based on length variation rather than pairwise alignments. Using this approach, we find a surprisingly strong relationship between difference in length and difference in the number of intrinsically disordered residues, where up to three quarters of the length variation can be explained by changes in the number of intrinsically disordered residues. Further, we find that disorder is common in both insertions and deletions. A more detailed analysis reveals that indel events do not induce disorder but rather that already disordered regions accrue indels, suggesting that there is a lowered selective pressure for indels to occur within intrinsically disordered regions.
Place, publisher, year, edition, pages
2013. Vol. 30, no 12, 2645-2653 p.
disordered proteins, insertions and deletions, indels, protein evolution, protein structure
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-98080DOI: 10.1093/molbev/mst157ISI: 000327793000010OAI: oai:DiVA.org:su-98080DiVA: diva2:682516
FunderSwedish Research Council, VR-NT 2009-5072Swedish Research Council, VR-M 2010-3555Swedish Research Council, VR-NT 2012-5046
SSF; Foundation for Strategic Research, Science for Life Laboratory; EU LSHG-CT-2004-503567 FP7-HEALTH-F4-2007-201924