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Recovery discrepancies of OH-PBDEs and polybromophenols in human plasma and cat serum versus herring and long-tailed duck plasma
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
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2014 (English)In: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 94, 97-103 p.Article in journal (Refereed) Published
Abstract [en]

Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) have been identified as metabolites of polybrominated diphenyl ethers (PBDEs) and/or as natural products. The OH-PBDEs and polybromophenols have come into focus over the last decade due to their abundance in biota and their potential adverse health effects. The present recovery study aims to validate a commonly used method (published by Hovander et al. 2000) for OH-PBDE analysis in human plasma. Further, the authors intended to determine the method's applicability to serum/plasma matrices from other species than humans. The investigated matrices were human plasma, cat serum, herring- and long-tailed duck plasma. The recovery study included nine OH-PBDEs, four polybromophenols and three methoxylated PBDEs (MeO-PBDEs). Five replicates of each matrix were spiked with these compounds at two dose levels; a low dose (0.5 ng) and a high dose (5 ng) and were cleaned up according to the Hovander method. The recovery of OH-PBDEs and polybromophenols in human plasma and cat serum were high and reproducible at both dose levels whereas the recovery for herring and long-tailed duck plasma were low and insufficient with great variability amongst OH-PBDE congeners at both dose levels. Our data show that the method can be fully applied to matrices like human plasma and cat serum but not for herring and long-tailed duck plasma without further method development. Hence care needs to be taken when applying the method onto other blood matrices without validation since the present study have demonstrated that the recoveries may differ amongst OH-PBDE congeners and specie.

Place, publisher, year, edition, pages
2014. Vol. 94, 97-103 p.
Keyword [en]
OH-PBDEs, Polybromophenols, Plasma, Serum, Recovery study
National Category
Chemical Sciences
Research subject
Environmental Chemistry
Identifiers
URN: urn:nbn:se:su:diva-98262DOI: 10.1016/j.chemosphere.2013.09.020ISI: 000327685300014OAI: oai:DiVA.org:su-98262DiVA: diva2:686152
Note

AuthorCount:5;

Available from: 2014-01-10 Created: 2014-01-03 Last updated: 2017-12-06Bibliographically approved
In thesis
1. Environmentally relevant chemical disruptors of oxidative phosphorylation in Baltic Sea biota: Exposure and toxic potentials
Open this publication in new window or tab >>Environmentally relevant chemical disruptors of oxidative phosphorylation in Baltic Sea biota: Exposure and toxic potentials
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis focuses on toxicity and occurrence of hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in Baltic Sea biota. The aims were to assess OH-PBDEs potency for disruption of oxidative phosphorylation (OXPHOS) and determine their and related compounds exposure in Baltic blue mussel, herring and long-tailed duck. A method for analysis of OH-PBDEs in herring and long-tailed duck plasma was also evaluated.

Relevant OH-PBDEs were tested in vitro for OXPHOS disruption, using a classic rat mitochondrial respiration assay and a cell mitochondrial membrane potential assay. All compounds were found to disrupt OXPHOS either by protonophoric uncoupling and/or via inhibition of the electron transport chain. 6-OH-BDE47 and 6-OH-BDE85, were identified as particularly potent OXPHOS disruptors. Strong synergism was observed when OH-PBDEs were tested as a mixture corresponding to what is present in Baltic blue mussels.

Baltic blue mussel is main feed for several species of mussel feeding sea ducks which have decreased dramatically in numbers. To assess long-tailed ducks exposure to brominated substances, liver tissue from long-tailed ducks wintering in the Baltic Sea and blue mussels were analysed. The result confirms that long-tailed duck are exposed to OH-PBDEs via their diet. However, low concentrations were found in the duck livers, which suggest low retention of these compounds despite daily intake. How the nutritional value of blue mussels as feed for sea ducks are affected by OH-PBDE exposure still needs further studies. Other species of sea ducks foraging on Baltic blue mussels during summer months can also be more exposed due to seasonal variation in primary production.

Herring sampled in the Baltic Proper and Bothnian Sea, were found to contain OH-PBDEs and high levels of their methylated counterpart, MeO-PBDEs. As demethylation of MeO-PBDEs is known to occur in fish, MeO-PBDEs may pose as additional source for more toxic OH-PBDEs in herring and their roe.

Place, publisher, year, edition, pages
Stockholm: Department of Environmental Science and Analytical Chemistry, Stockholm University, 2015. 69 p.
Keyword
Oxidative phosphorylation, OH-PBDEs, MeO-PBDEs, PBDEs, long-tailed duck (Clangula hyemalis), Baltic herring (Clupea harengus), Baltic blue mussel (Mytilus trossulus x Mytilus edulis), Baltic Sea
National Category
Chemical Sciences
Research subject
Environmental Chemistry
Identifiers
urn:nbn:se:su:diva-115436 (URN)978-91-7649-127-0 (ISBN)
Public defence
2015-05-13, Magnéli Hall, Arrhenius Laboratory, Svante Arrhenius väg 16 B, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 2: Manuscript. Paper 3: Manuscript.

Available from: 2015-04-21 Created: 2015-03-24 Last updated: 2015-04-21Bibliographically approved
2. Cats as a biomarker for exposure to POPs in home environments: – with focus on brominated chemicals and associations to feline hyperthyroidism
Open this publication in new window or tab >>Cats as a biomarker for exposure to POPs in home environments: – with focus on brominated chemicals and associations to feline hyperthyroidism
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis, body burden of brominated chemicals such as polybrominated diphenyl ethers (PBDEs) and brominated phenolic substances are explored. The external exposure of cats to these compounds from house dust and their food was also investigated. The analytical methodology used for serum extractions was validated for analysis of OH-PBDEs in cat serum.

Cats are highly exposed to dust and thereby also to chemicals accumulated in dust, due to their grooming behavior. This makes pet cats, a suitable biomarker for exposure to chemicals in house dust in home environments. Thereby, cats’ exposure to dust is somewhat similar to toddlers, due their hand-to-mouth behavior. Thus, cats’ internal exposure may be used to access that of toddlers. The PBDE pattern in Swedish pet cats show exposure to Penta-, Octa- and DecaBDE and their profile matches that of dust from their homes, suggesting dust to be an important exposure source. Serum concentrations of BDE-47 in the cats were also shown to correlate with house dust from their living rooms.

Feline hyperthyroidism (FH) is a common endocrine disease in elderly cats worldwide, still the actual cause(s) has not been established even though environmental pollutants such as PBDEs have been suggested. Difference in contamination load between cats with normal thyroid status and cats diagnosed with FH was performed and higher serum concentrations for some PBDEs (BDE-99, 153, -183) were found in the hyperthyroid cats.

Further, the presence of the longtime discontinued flame retardant, decabromobiphenyl (BB-209) in all sampled cats indicates that it is still being circulated. A significant correlation between serum concentrations of BB-209 and matched cat food samples was found.

Very few OH-PBDEs were indeed shown in cat serum and the dominating compound, 6-OH-BDE47, is believed to be of natural origin rather than being a metabolite of BDE-47. A significant correlation between serum concentration of 6-OH-BDE47 and cat food was found.

Place, publisher, year, edition, pages
Stockholm: Department of Environmental Science and Analytical Chemistry, Stockholm University, 2015. 74 p.
National Category
Other Chemistry Topics
Research subject
Environmental Chemistry
Identifiers
urn:nbn:se:su:diva-116904 (URN)978-91-7649-190-4 (ISBN)
Public defence
2015-06-11, Magnéli Hall, Arrhenius Laboratory, Svante Arrhenius väg 16 B, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

At the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 3: Manuscript.

Available from: 2015-05-20 Created: 2015-05-04 Last updated: 2015-06-24Bibliographically approved

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Dahlberg, Anna-KarinNorrgran, JessicaBergman, ÅkeAsplund, Lillemor
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