Peptide-Based Delivery of Oligonucleotides Across Blood-Brain Barrier Model
2014 (English)In: International Journal of Peptide Research and Therapeutics, ISSN 1573-3904, Vol. 20, no 2, 169-178 p.Article in journal (Refereed) Published
Delivery of pharmaceutical agents across a blood–brain barrier (BBB) is a challenge for brain cancer therapy. In this study, an in vitro BBB model was utilized to study the delivery of oligonucleotides across brain endothelial cells targeting to glioma cells in a Transwell™ setup. A series of novel peptides were synthesized by covalent conjugation of cell-penetrating peptides with targeting peptides for delivery of gene-based therapeutics. These peptides were screened for passage across the Transwell™ and we found the most efficient peptide PepFect32 from originating PepFect 14 coupled with the targeting peptide angiopep-2. PepFect32/pDNA nanocomplexes exhibited high transcytosis across the BBB in vitro model and the highest transfection efficiency to glioma cells. In conclusion, PepFect32 revealed the most efficient peptide-based vector for pDNA delivery across in vitro BBB model.
Place, publisher, year, edition, pages
2014. Vol. 20, no 2, 169-178 p.
Blood–brain barrier model, Cell-penetrating peptide, bEnd.3, Glioma cells, Plasmid transfection, Gene-based therapy
Research subject Neurochemistry with Molecular Neurobiology
IdentifiersURN: urn:nbn:se:su:diva-100010DOI: 10.1007/s10989-013-9378-4ISI: 000334420100007OAI: oai:DiVA.org:su-100010DiVA: diva2:690404