Change search
ReferencesLink to record
Permanent link

Direct link
Rrp5 Binding at Multiple Sites Coordinates Pre-rRNA Processing and Assembly
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Show others and affiliations
2013 (English)In: Molecular Cell, ISSN 1097-2765, E-ISSN 1097-4164, Vol. 52, no 5, 707-719 p.Article in journal (Refereed) Published
Abstract [en]

In vivo UV crosslinking identified numerous preribosomal RNA (pre-rRNA) binding sites for the large, highly conserved ribosome synthesis factor Rrp5. Intramolecular complementation has shown that the C-terminal domain (CTD) of Rrp5 is required for pre-rRNA cleavage at sites A0-A2 on the pathway of 18S rRNA synthesis, whereas the N-terminal domain (NTD) is required for A3 cleavage on the pathway of 5.8S/25S rRNA synthesis. The CTD was crosslinked to sequences flanking A2 and to the snoRNAs U3, U14, snR30, and snR10, which are required for cleavage at A0-A2. The NTD was crosslinked to sequences flanking A3 and to the RNA component of ribonuclease MRP, which cleaves site A3. Rrp5 could also be directly crosslinked to several large structural proteins and nucleoside triphosphatases. A key role in coordinating preribosomal assembly and processing was confirmed by chromatin spreads. Following depletion of Rrp5, cotranscriptional cleavage was lost and preribosome compaction greatly reduced.

Place, publisher, year, edition, pages
2013. Vol. 52, no 5, 707-719 p.
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:su:diva-99883DOI: 10.1016/j.molcel.2013.10.017ISI: 000328591800010OAI: diva2:690468


Funding agencies:

Wellcome Trust, 077248, 084229, 091020, 092076; National Institute of General Medical Sciences; National Institutes of Health, RO1-GM63952; Swedish Research Fund; Carl Tryggers Stiftelse;  EMBO 

Available from: 2014-01-23 Created: 2014-01-20 Last updated: 2014-01-23Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Wieslander, Lars
By organisation
Department of Molecular Biosciences, The Wenner-Gren Institute
In the same journal
Molecular Cell
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 217 hits
ReferencesLink to record
Permanent link

Direct link