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HiRIEF LC-MSMS enables deep proteome coverage and unbiased proteogenomics
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
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2014 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 11, no 1, 59-+ p.Article in journal (Refereed) Published
Abstract [en]

We present a liquid chromatography-mass spectrometry (LC-MSMS)-based method permitting unbiased (gene prediction-independent) genome-wide discovery of protein-coding loci in higher eukaryotes. Using high-resolution isoelectric focusing (HiRIEF) at the peptide level in the 3.7-5.0 pH range and accurate peptide isoelectric point (pI) prediction, we probed the six-reading-frame translation of the human and mouse genomes and identified 98 and 52 previously undiscovered protein-coding loci, respectively. The method also enabled deep proteome coverage, identifying 13,078 human and 10,637 mouse proteins.

Place, publisher, year, edition, pages
2014. Vol. 11, no 1, 59-+ p.
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Biochemistry and Molecular Biology
URN: urn:nbn:se:su:diva-100102DOI: 10.1038/NMETH.2732ISI: 000329178200027OAI: diva2:691938


Available from: 2014-01-29 Created: 2014-01-27 Last updated: 2014-01-29Bibliographically approved

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Granholm, ViktorHuss, Mikael
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Department of Biochemistry and BiophysicsScience for Life Laboratory (SciLifeLab)
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