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Sensitive and Rapid Detection of Chlamydia trachomatis by Recombinase Polymerase Amplification Directly from Urine Samples
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2014 (English)In: Journal of Molecular Diagnostics, ISSN 1525-1578, E-ISSN 1943-7811, Vol. 16, no 1, 127-135 p.Article in journal (Refereed) Published
Abstract [en]

Chlamydia trachomatis is the most common sexually transmitted human pathogen. Infection results in minimal to no symptoms in approximately two-thirds of women and therefore often goes undiagnosed. C. trachomatis infections are a major public health concern because of the potential severe long-term consequences, including an increased risk of ectopic pregnancy, chronic pelvic pain, and infertility. To date, several point-of-care tests have been developed for C. trachomatis diagnostics. Although many of them are fast and specific, they lack the required sensitivity for large-scale application. We describe a rapid and sensitive form of detection directly from urine samples. The assay uses recombinase polymerase amplification and has a minimum detection limit of 5 to 12 pathogens per test. Furthermore, it enables detection within 20 minutes directly from urine samples without DNA purification before the amplification reaction. Initial analysis of the assay from clinical patient samples had a specificity of 100% (95% CI, 92%-100%) and a sensitivity of 83% (95% CI, 51%-97%). The whole procedure is fairly simple and does not require specific machinery, making it potentially applicable in point-of-care settings.

Place, publisher, year, edition, pages
2014. Vol. 16, no 1, 127-135 p.
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Biochemistry and Molecular Biology
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URN: urn:nbn:se:su:diva-100098DOI: 10.1016/j.jmoldx.2013.08.003ISI: 000328926500014OAI: oai:DiVA.org:su-100098DiVA: diva2:692337
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AuthorCount:10;

Available from: 2014-01-30 Created: 2014-01-27 Last updated: 2017-12-06Bibliographically approved

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Langel, Ülo
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