Interaction between Transcription Factors of E2F family and DNA Studied with Infrared Spectroscopy
(English)Manuscript (preprint) (Other academic)
The E2F transcription factors are crucial for the regulation of genes required for proper progression through the cell cycle. Since E2Fs can initiate both cell proliferation and cell death, it is not surprising that these proteins are the subjects of extensive studies. In this work we characterize the formation of E2F1- and E2F8-DNA complexes with Fourier transform infrared spectroscopy. We demonstrate the changes in the secondary structure of the E2F1 and E2F8, in particular the disappearance of regular α-helices. We also show the perturbation to the DNA double helix and characterize the interactions between the amino acids in the proteins and the bases in the DNA.
IdentifiersURN: urn:nbn:se:su:diva-101086OAI: oai:DiVA.org:su-101086DiVA: diva2:698803