Identification of novel sphingolipid-binding motifs in mammalian membrane proteins
(English)Manuscript (preprint) (Other academic)
Specific interactions between transmembrane proteins and sphingolipids is a poorly understood phenomenon, and only a couple of instances have been identified. The best characterized example is the sphingolipid-binding motif VXXTLXXIY found in the transmembrane helix of the vesicular transport protein p24. Here, we have used a simple motif- probability algorithm (MOPRO) to identify proteins that contain putative sphingolipid-binding motifs in a dataset comprising full proteomes from mammalian organisms. Four selected candidate proteins all tested positive for sphingolipid binding in a photoaffinity assay. The putative sphingolipid-binding motifs are noticeably enriched in the 7TM family of G-protein coupled receptors, predominantly in transmembrane helix 6.
sphingolipid;, transmembrane helix
Bioinformatics (Computational Biology)
Research subject Biochemistry towards Bioinformatics
IdentifiersURN: urn:nbn:se:su:diva-101791OAI: oai:DiVA.org:su-101791DiVA: diva2:705529