The Escherichia coli envelope stress sensor CpxA can sense changes in lipid bilayer properties
(English)Manuscript (preprint) (Other academic)
Membrane proteins are evolutionarily integrated with their lipid bilayer environment, but it is only marginally understood how they sense and adapt to changes in lipid composition. Here we use an in vitro reconstitution system to demonstrate that the activity of envelope stress sensor CpxA of Escherichia coli is modulated by lipid bilayer properties. By changing the lipid composition of the vesicles, and using lipid-engineered E. coli strains we show that CpxA activity is regulated by means of different properties of the bilayer: (i) anionic surface charge (ii) membrane curvature (iii) thus lateral bilayer stress but also (iv) membrane bilayer thickness. We conclude that the CpxA could sense changes in the lipid bilayer properties.
CpxA envelope stress sensor, lipid bilayer stress, lipid-engineered E. coli strains, surface charge, lateral stress, membrane curvature, bilayer thickness
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:su:diva-102676OAI: oai:DiVA.org:su-102676DiVA: diva2:712364
FunderSwedish Research Council