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Contrasting effects of cold acclimation versus obesogenic diets onchemerin gene expression in brown and brite adipose tissues
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Based on results from a signal sequence trap, we investigated chemerin gene expression in brown adipose tissue. Male NMRI mice were exposed to 30, 22 or 4 °C for 3 weeks, or were fed control (chow) diet, cafeteria diet or high-fat diet at thermoneutrality for the same time. In brown adipose tissue, cold acclimation strongly diminished chemerin gene expression, whereas obesogenic diets augmented expression. Qualitatively, changes in expression were paralleled in brite/beige adipose tissues (e.g. inguinal), whereas white adipose tissue (epididymal) did not react to these cues. Changes in tissue expression were not paralleled by alterations in plasma levels. The cellular regulation was not congruent with a sympathetic/adrenergic control of expression. The data are discussed in relation to suggested endocrine, paracrine and autocrine effects of this adipokine.

National Category
Natural Sciences Biochemistry and Molecular Biology
Research subject
Physiology
Identifiers
URN: urn:nbn:se:su:diva-102923OAI: oai:DiVA.org:su-102923DiVA: diva2:714060
Available from: 2014-04-25 Created: 2014-04-25 Last updated: 2014-04-25
In thesis
1. The secretome of brown adipose tissue
Open this publication in new window or tab >>The secretome of brown adipose tissue
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Brown adipose tissue has long been known for its heat-producing capacity, but less is known about its possible effects as a secretory organ. This thesis summarizes information about presently known factors secreted from brown adipose tissue and about their actions. We were able to add factors to the list by the use of a signal-sequence trap method. Results from the signal-sequence trap generated a list of suggested brown adipocyte secreted proteins; gene expression of these proteins was then further studied with microarray technique.

One of the genes further analyzed was the adipokine chemerin. Gene expression of chemerin in brown adipose tissue was decreased in cold acclimation but increased with a high-caloric diet. This indicates that factors other than norepinephrine influence chemerin gene expression. The effects on chemerin gene expression were not be reflected in serum levels; therefore, chemerin secreted from brown adipose tissue is ascribed an autocrine/paracrine role.

Signal-sequence trap and microarray studies suggested adrenomedullin, collagen type 3 a1, lipocalin 2 and Niemann Pick type C2 to be highly secreted from brown adipocytes. Gene expression of these factors was examined in vivo and in vitro. Our studies showed that both cold acclimation and high-caloric diet have an effect on gene expression of these factors. However, there was no effect on gene expression of chemerin and collagen type 3 a1 in norepinephrine-treated brown adipocyte cell cultures. This suggests that effects on gene expression of the examined possible brown adipocyte secreted proteins are not solely controlled by norepinephrine.

Place, publisher, year, edition, pages
Stockholm: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 2014. 101 p.
National Category
Biochemistry and Molecular Biology
Research subject
Physiology
Identifiers
urn:nbn:se:su:diva-102934 (URN)978-91-7447-903-4 (ISBN)
Public defence
2014-05-28, Lecture hall E306, Arrheniuslaboratorierna, Svante Arrhenius väg 20 C, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

At the time of doctoral defence the following papers were unpublished and had a status as follows: Paper 1: Manuscript; Paper 3: Manuscript; Paper  4: Manuscript; Paper 5: Manuscript

Available from: 2014-05-06 Created: 2014-04-25 Last updated: 2014-04-28Bibliographically approved

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