A partial secretome of brown adipose tissue
(English)Manuscript (preprint) (Other academic)
To identify brown adipocyte secreted proteins a signal-sequence trap method was used. All genes identified were cloned and studied with microarray technique.
The aim of this study was to evaluate how these genes were influenced under different physiological conditions, both
in vivo and in vitro. Microarray studies were performed comparing primary brown adipocytes stimulated with norepinephrine to non-stimulated, and primary brown adipocytes compared to primary white adipocytes. In vivo studies were performed to evaluate physiological effects on gene expression in brown adipose tissue. Male NMRI mice were placed in cold or at thermoneutrality for 3 weeks and compared. Mice kept at room temperature were exposed to cafeteria diet for three weeks compared to regular diet.
Results show that norepinephrine had effects on the expression of these potentially secreted genes. However, gene expression from physiological studies
in vivo that could be expected to show similar expression patterns as norepinephrine-treated brown adipocytes did not do so. This indicates that other factors than norepinephrine can regulate gene expression of possible brown adipose tissue-secreted factors.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-102928OAI: oai:DiVA.org:su-102928DiVA: diva2:714062