Physiological effects on gene expression of some brown adipose tissue secreted factors
(English)Manuscript (preprint) (Other academic)
Background: To investigate the role of brown adipose tissue as a secretory organ, a signal-sequence trap and microarray study were performed. Results indicated that adrenomedullin, collagen type 3 a1, lipocalin 2 and Niemann Pick type C 2 could be possible brown adipocyte-secreted proteins.
Method: To investigate the physiological regulation of the gene expression of the adrenomedullin, collagen 3 a1, lipocalin 2 and Niemann Pick type C2 in brown adipose tissue, the following study was performed. Mice were exposed to cold or different high-caloric diets (high-fat diet and cafeteria diet) for three weeks and qPCR studies were performed of the genes of interest.
Results & Conclusion: The study indicates that cold acclimation significantly suppressed gene expression of adrenomedullin, collagen type 3a and lipocalin 2 (P < 0.05). There was also an effect of the different diets but the results were not cohesive between the two high-caloric diets. However, none of the expression patterns between the cold-acclimated mice or the diet studies overlapped, suggesting that norepinephrine is not the key regulator of the gene expression of any of the investigated genes
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-102932OAI: oai:DiVA.org:su-102932DiVA: diva2:714069