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Physiological effects on gene expression of some brown adipose tissue secreted factors
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Background: To investigate the role of brown adipose tissue as a secretory organ, a signal-sequence trap and microarray study were performed. Results indicated that adrenomedullin, collagen type 3 a1, lipocalin 2 and Niemann Pick type C 2 could be possible brown adipocyte-secreted proteins.

Method: To investigate the physiological regulation of the gene expression of the adrenomedullin, collagen 3 a1, lipocalin 2 and Niemann Pick type C2 in brown adipose tissue, the following study was performed. Mice were exposed to cold or different high-caloric diets (high-fat diet and cafeteria diet) for three weeks and qPCR studies were performed of the genes of interest.

Results & Conclusion: The study indicates that cold acclimation significantly suppressed gene expression of adrenomedullin, collagen type 3a and lipocalin 2 (P < 0.05). There was also an effect of the different diets but the results were not cohesive between the two high-caloric diets. However, none of the expression patterns between the cold-acclimated mice or the diet studies overlapped, suggesting that norepinephrine is not the key regulator of the gene expression of any of the investigated genes

National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-102932OAI: oai:DiVA.org:su-102932DiVA: diva2:714069
Available from: 2014-04-25 Created: 2014-04-25 Last updated: 2014-04-25
In thesis
1. The secretome of brown adipose tissue
Open this publication in new window or tab >>The secretome of brown adipose tissue
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Brown adipose tissue has long been known for its heat-producing capacity, but less is known about its possible effects as a secretory organ. This thesis summarizes information about presently known factors secreted from brown adipose tissue and about their actions. We were able to add factors to the list by the use of a signal-sequence trap method. Results from the signal-sequence trap generated a list of suggested brown adipocyte secreted proteins; gene expression of these proteins was then further studied with microarray technique.

One of the genes further analyzed was the adipokine chemerin. Gene expression of chemerin in brown adipose tissue was decreased in cold acclimation but increased with a high-caloric diet. This indicates that factors other than norepinephrine influence chemerin gene expression. The effects on chemerin gene expression were not be reflected in serum levels; therefore, chemerin secreted from brown adipose tissue is ascribed an autocrine/paracrine role.

Signal-sequence trap and microarray studies suggested adrenomedullin, collagen type 3 a1, lipocalin 2 and Niemann Pick type C2 to be highly secreted from brown adipocytes. Gene expression of these factors was examined in vivo and in vitro. Our studies showed that both cold acclimation and high-caloric diet have an effect on gene expression of these factors. However, there was no effect on gene expression of chemerin and collagen type 3 a1 in norepinephrine-treated brown adipocyte cell cultures. This suggests that effects on gene expression of the examined possible brown adipocyte secreted proteins are not solely controlled by norepinephrine.

Place, publisher, year, edition, pages
Stockholm: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 2014. 101 p.
National Category
Biochemistry and Molecular Biology
Research subject
Physiology
Identifiers
urn:nbn:se:su:diva-102934 (URN)978-91-7447-903-4 (ISBN)
Public defence
2014-05-28, Lecture hall E306, Arrheniuslaboratorierna, Svante Arrhenius väg 20 C, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

At the time of doctoral defence the following papers were unpublished and had a status as follows: Paper 1: Manuscript; Paper 3: Manuscript; Paper  4: Manuscript; Paper 5: Manuscript

Available from: 2014-05-06 Created: 2014-04-25 Last updated: 2014-04-28Bibliographically approved

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