The possibility that brown adipose tissue can secrete factors to affect overall metabolism is a subject that attracts attention. Previous studies from our laboratory suggest that chemerin and collagen type III a1 (Col3a1) could be interesting factors secreted from brown adipose tissue.
To evaluate chemerin and Col3a1 gene expression regulation, primary brown adipocyte cultures were stimulated with norepinephrine at different stages of differentiation. The present study shows that there was an effect of differentiation on gene expression of these factors but there was no effect of norepinephrine treatment.
There has been a suggestion that different adenylate cyclases are responsible for regulating gene expression in brown adipocytes. However, neither differentiation nor norepinephrine treatment affected adenylate cyclase gene expression dramatically.
The results suggest that norepinephrine is not the main regulator of gene expression of either of the two possible brown adipocyte-secreted factors, chemerin and Col3a1. Surprisingly, norepinephrine had no major effect on adenylate cyclase expression at any stage of differentiation.