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Effects of differentiation on gene expression of certain brown adipocyte-secreted factors
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

The possibility that brown adipose tissue can secrete factors to affect overall metabolism is a subject that attracts attention. Previous studies from our laboratory suggest that chemerin and collagen type III a1 (Col3a1) could be interesting factors secreted from brown adipose tissue.

To evaluate chemerin and Col3a1 gene expression regulation, primary brown adipocyte cultures were stimulated with norepinephrine at different stages of differentiation. The present study shows that there was an effect of differentiation on gene expression of these factors but there was no effect of norepinephrine treatment.

There has been a suggestion that different adenylate cyclases are responsible for regulating gene expression in brown adipocytes. However, neither differentiation nor norepinephrine treatment affected adenylate cyclase gene expression dramatically.

The results suggest that norepinephrine is not the main regulator of gene expression of either of the two possible brown adipocyte-secreted factors, chemerin and Col3a1. Surprisingly, norepinephrine had no major effect on adenylate cyclase expression at any stage of differentiation.

National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-102933OAI: oai:DiVA.org:su-102933DiVA: diva2:714070
Available from: 2014-04-25 Created: 2014-04-25 Last updated: 2014-04-25
In thesis
1. The secretome of brown adipose tissue
Open this publication in new window or tab >>The secretome of brown adipose tissue
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Brown adipose tissue has long been known for its heat-producing capacity, but less is known about its possible effects as a secretory organ. This thesis summarizes information about presently known factors secreted from brown adipose tissue and about their actions. We were able to add factors to the list by the use of a signal-sequence trap method. Results from the signal-sequence trap generated a list of suggested brown adipocyte secreted proteins; gene expression of these proteins was then further studied with microarray technique.

One of the genes further analyzed was the adipokine chemerin. Gene expression of chemerin in brown adipose tissue was decreased in cold acclimation but increased with a high-caloric diet. This indicates that factors other than norepinephrine influence chemerin gene expression. The effects on chemerin gene expression were not be reflected in serum levels; therefore, chemerin secreted from brown adipose tissue is ascribed an autocrine/paracrine role.

Signal-sequence trap and microarray studies suggested adrenomedullin, collagen type 3 a1, lipocalin 2 and Niemann Pick type C2 to be highly secreted from brown adipocytes. Gene expression of these factors was examined in vivo and in vitro. Our studies showed that both cold acclimation and high-caloric diet have an effect on gene expression of these factors. However, there was no effect on gene expression of chemerin and collagen type 3 a1 in norepinephrine-treated brown adipocyte cell cultures. This suggests that effects on gene expression of the examined possible brown adipocyte secreted proteins are not solely controlled by norepinephrine.

Place, publisher, year, edition, pages
Stockholm: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 2014. 101 p.
National Category
Biochemistry and Molecular Biology
Research subject
Physiology
Identifiers
urn:nbn:se:su:diva-102934 (URN)978-91-7447-903-4 (ISBN)
Public defence
2014-05-28, Lecture hall E306, Arrheniuslaboratorierna, Svante Arrhenius väg 20 C, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

At the time of doctoral defence the following papers were unpublished and had a status as follows: Paper 1: Manuscript; Paper 3: Manuscript; Paper  4: Manuscript; Paper 5: Manuscript

Available from: 2014-05-06 Created: 2014-04-25 Last updated: 2014-04-28Bibliographically approved

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