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Avoiding pitfalls in gene (co)expression meta-analysis
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab). Swedish e-Science Research Centre, Sweden.
2014 (English)In: Genomics, ISSN 0888-7543, E-ISSN 1089-8646, Vol. 103, no 1, 21-30 p.Article in journal (Refereed) Published
Abstract [en]

Differential gene expression analysis between healthy and diseased groups is a widely used approach to understand the molecular underpinnings of disease. A wide variety of experimental and bioinformatics techniques are available for this type of analysis, yet their impact on the reliability of the results has not been systematically studied. We performed a large scale comparative analysis of clinical expression data, using several background corrections and differential expression metrics. The agreement between studies was analyzed for study pairs of same cancer type, of different cancer types, and between cancer and non-cancer studies. We also replicated the analysis using differential coexpression. We found that agreement of differential expression is primarily dictated by the microarray platform, while differential coexpression requires large sample sizes. Two studies using different differential expression metrics may show no agreement, even if they agree strongly using the same metric. Our analysis provides practical recommendations for gene (co)expression analysis.

Place, publisher, year, edition, pages
2014. Vol. 103, no 1, 21-30 p.
Keyword [en]
Differential expression, Differential coexpression, Cancer gene expression, Microarray data processing
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-102993DOI: 10.1016/j.ygeno.2013.10.006ISI: 000333512800003OAI: oai:DiVA.org:su-102993DiVA: diva2:714212
Note

AuthorCount:2;

Available from: 2014-04-25 Created: 2014-04-25 Last updated: 2017-12-05Bibliographically approved

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Östlund, GabrielSonnhammer, Erik L. L.
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