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Molecularly imprinted polymer cartridges coupled on-line with high performance liquid cheomatography for simple and rapid analysis of dextromethorphan in human plasma samples
Amirkabir University of Technology, Hafez, Tehran, Iran.
Amirkabir University of Technology, Hafez, Tehran, Iran.
Ministry of Health and Medical Education, Tehran, Iran.
2011 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 879, no 11-12, 777-782 p.Article in journal (Refereed) Published
Abstract [en]

In this paper, a novel method is described for automated determination of dextromethorphan in biological fluids using molecularly imprinted solid-phase extraction (MISPE) as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and dextromethorphan as template molecule. These imprinted polymers were used as solid-phase extraction sorbent for the extraction of dextromethorphan from human plasma samples. Various parameters affecting the extraction efficiency of the MIP cartridges were evaluated. The high selectivity of the sorbent coupled to the high performance liquid chromatographic system permitted a simple and rapid analysis of this drug in plasma samples with limits of detection (LOD) and quantification (LOQ) of 0.12 ng/mL and 0.35 ng/mL, respectively. The MIP selectivity was evaluated by analyzing of the dextromethorphan in presence of several substances with similar molecular structures and properties. Results from the HPLC analyses showed that the recoveries of dextromethorphan using MIP cartridges from human plasma samples in the range of 1–50 ng/mL were higher than 87%.

Place, publisher, year, edition, pages
2011. Vol. 879, no 11-12, 777-782 p.
Keyword [en]
Molecularly imprinted polymer, Dextromethorphan, Human plasma, Automated, Sample clean-up
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-103346DOI: 10.1016/j.jchromb.2011.02.031OAI: oai:DiVA.org:su-103346DiVA: diva2:716766
Available from: 2014-05-12 Created: 2014-05-12 Last updated: 2017-12-05Bibliographically approved
In thesis
1. Towards more selective sorbents for extraction of drugs and biomarkers from biological fluids using molecularly imprinted polymers
Open this publication in new window or tab >>Towards more selective sorbents for extraction of drugs and biomarkers from biological fluids using molecularly imprinted polymers
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Sample preparation has a critical role as a first step in analytical processes, especially in bioanalysis and environmental analysis. A good sample preparation technique should be robust and stable, regardless of the sample matrix.

The aim of this thesis is to design and synthesize molecularly imprinted polymers that can be used in various sample preparation techniques, such as on-line MEPS, on-line SPE and on-line monolithic pre-columns used for the extraction of drugs, hormones, and cancer biomarkers from human plasma and urine samples. Additional aim was to provide full automation, on-line coupling, short sample preparation time and high-throughput.

In this thesis MIP in MEPS was used on-line with liquid chromatography-tandem mass spectrometry (LC/MS/MS) for the determination of sarcosine in human urine and plasma samples. The method was fully automated and the packed sorbent could be used for about hundred extractions. In additional work a coated needle with MIP-Sol-Gel as thin layer was prepared and used for the microextraction of bilirubin from human plasma and urine. Small sample volumes could be handled and the validation of the method showed that the method was robust and selective.

In a further work MIP-SPE on-line with HPLC was used for the extraction and determination of dextromethorphan in human plasma samples. MIP-SPE showed a good selectivity and high recovery (87% - 92%). On-line MIP monolithic pre-column was prepared and used in a coupled system for the extraction of tramadol in human plasma and urine samples. The MIP monolithic pre-column showed good selectivity and high extraction recovery was obtained (91-96%). The extraction and analysis of human insulin in plasma and pharmaceutical formulation solutions were carried out using MIP-SPE on-line with HPLC. The validation of the method showed that the method was accurate and robust.

Place, publisher, year, edition, pages
Stockholm: Department of Analytical Chemistry, Stockholm University, 2014. 77 p.
Keyword
molecularly imprinted polymer, sol-gel, solid phase extraction, microextraction by packed sorbent, monolithic column
National Category
Chemical Sciences
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-103379 (URN)978-91-7447-904-1 (ISBN)
Public defence
2014-06-12, G-salen, Arrheniuslaboratorierna, Svante Arrhenius väg 20 C, Stockholm, 13:00 (English)
Opponent
Supervisors
Note

At the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 5: Submitted.

Available from: 2014-05-22 Created: 2014-05-13 Last updated: 2014-05-20Bibliographically approved

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