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Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of human insulin in plasma and pharmaceutical formulations
Amirkabir University of Technology, Tehran, Iran.
Amirkabir University of Technology, Tehran, Iran.
Ministry of Health and Medical Education, Tehran, Iran.
2014 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 121, 30-36 p.Article in journal (Refereed) Published
Abstract [en]

In this paper, a novel method is described for automated determination of human insulin in biological fluids using principle of sequential injection on a molecularly imprinted solid-phase extraction (MISPE) cartridge as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, chloroform as a porogen and insulin as a template molecule. The imprinted polymers were then employed as the solid-phase extraction sorbent for on-line extraction of insulin from human plasma samples. To achieve the best condition, influential parameters on the extraction efficiency were thoroughly investigated. Rapid and simple analysis of the hormone was successfully accomplished through the good selectivity of the prepared sorbent coupled with HPLC. Limits of detection (LOD) and quantification (LOQ) of 0.2 ng mL−1, 0.7 ng mL−1, and 0.03 ng mL−1, 0.1 ng mL−1 were obtained in plasma and urine respectively. The obtained data exhibited the great recoveries for extraction of insulin from human plasma and pharmaceutical samples, higher than 87%.

Place, publisher, year, edition, pages
2014. Vol. 121, 30-36 p.
Keyword [en]
Molecularly imprinted polymer, Insulin, Human plasma, Pharmaceutical formulations, Automated, Sample clean-up
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-103355DOI: 10.1016/j.talanta.2013.12.011OAI: oai:DiVA.org:su-103355DiVA: diva2:716769
Available from: 2014-05-12 Created: 2014-05-12 Last updated: 2017-12-05Bibliographically approved
In thesis
1. Towards more selective sorbents for extraction of drugs and biomarkers from biological fluids using molecularly imprinted polymers
Open this publication in new window or tab >>Towards more selective sorbents for extraction of drugs and biomarkers from biological fluids using molecularly imprinted polymers
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Sample preparation has a critical role as a first step in analytical processes, especially in bioanalysis and environmental analysis. A good sample preparation technique should be robust and stable, regardless of the sample matrix.

The aim of this thesis is to design and synthesize molecularly imprinted polymers that can be used in various sample preparation techniques, such as on-line MEPS, on-line SPE and on-line monolithic pre-columns used for the extraction of drugs, hormones, and cancer biomarkers from human plasma and urine samples. Additional aim was to provide full automation, on-line coupling, short sample preparation time and high-throughput.

In this thesis MIP in MEPS was used on-line with liquid chromatography-tandem mass spectrometry (LC/MS/MS) for the determination of sarcosine in human urine and plasma samples. The method was fully automated and the packed sorbent could be used for about hundred extractions. In additional work a coated needle with MIP-Sol-Gel as thin layer was prepared and used for the microextraction of bilirubin from human plasma and urine. Small sample volumes could be handled and the validation of the method showed that the method was robust and selective.

In a further work MIP-SPE on-line with HPLC was used for the extraction and determination of dextromethorphan in human plasma samples. MIP-SPE showed a good selectivity and high recovery (87% - 92%). On-line MIP monolithic pre-column was prepared and used in a coupled system for the extraction of tramadol in human plasma and urine samples. The MIP monolithic pre-column showed good selectivity and high extraction recovery was obtained (91-96%). The extraction and analysis of human insulin in plasma and pharmaceutical formulation solutions were carried out using MIP-SPE on-line with HPLC. The validation of the method showed that the method was accurate and robust.

Place, publisher, year, edition, pages
Stockholm: Department of Analytical Chemistry, Stockholm University, 2014. 77 p.
Keyword
molecularly imprinted polymer, sol-gel, solid phase extraction, microextraction by packed sorbent, monolithic column
National Category
Chemical Sciences
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-103379 (URN)978-91-7447-904-1 (ISBN)
Public defence
2014-06-12, G-salen, Arrheniuslaboratorierna, Svante Arrhenius väg 20 C, Stockholm, 13:00 (English)
Opponent
Supervisors
Note

At the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 5: Submitted.

Available from: 2014-05-22 Created: 2014-05-13 Last updated: 2014-05-20Bibliographically approved

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