Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Towards more selective sorbents for extraction of drugs and biomarkers from biological fluids using molecularly imprinted polymers
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Sample preparation has a critical role as a first step in analytical processes, especially in bioanalysis and environmental analysis. A good sample preparation technique should be robust and stable, regardless of the sample matrix.

The aim of this thesis is to design and synthesize molecularly imprinted polymers that can be used in various sample preparation techniques, such as on-line MEPS, on-line SPE and on-line monolithic pre-columns used for the extraction of drugs, hormones, and cancer biomarkers from human plasma and urine samples. Additional aim was to provide full automation, on-line coupling, short sample preparation time and high-throughput.

In this thesis MIP in MEPS was used on-line with liquid chromatography-tandem mass spectrometry (LC/MS/MS) for the determination of sarcosine in human urine and plasma samples. The method was fully automated and the packed sorbent could be used for about hundred extractions. In additional work a coated needle with MIP-Sol-Gel as thin layer was prepared and used for the microextraction of bilirubin from human plasma and urine. Small sample volumes could be handled and the validation of the method showed that the method was robust and selective.

In a further work MIP-SPE on-line with HPLC was used for the extraction and determination of dextromethorphan in human plasma samples. MIP-SPE showed a good selectivity and high recovery (87% - 92%). On-line MIP monolithic pre-column was prepared and used in a coupled system for the extraction of tramadol in human plasma and urine samples. The MIP monolithic pre-column showed good selectivity and high extraction recovery was obtained (91-96%). The extraction and analysis of human insulin in plasma and pharmaceutical formulation solutions were carried out using MIP-SPE on-line with HPLC. The validation of the method showed that the method was accurate and robust.

Place, publisher, year, edition, pages
Stockholm: Department of Analytical Chemistry, Stockholm University , 2014. , 77 p.
Keyword [en]
molecularly imprinted polymer, sol-gel, solid phase extraction, microextraction by packed sorbent, monolithic column
National Category
Chemical Sciences
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-103379ISBN: 978-91-7447-904-1 (print)OAI: oai:DiVA.org:su-103379DiVA: diva2:716857
Public defence
2014-06-12, G-salen, Arrheniuslaboratorierna, Svante Arrhenius väg 20 C, Stockholm, 13:00 (English)
Opponent
Supervisors
Note

At the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 5: Submitted.

Available from: 2014-05-22 Created: 2014-05-13 Last updated: 2014-05-20Bibliographically approved
List of papers
1. Molecularly imprinted polymer cartridges coupled on-line with high performance liquid cheomatography for simple and rapid analysis of dextromethorphan in human plasma samples
Open this publication in new window or tab >>Molecularly imprinted polymer cartridges coupled on-line with high performance liquid cheomatography for simple and rapid analysis of dextromethorphan in human plasma samples
2011 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 879, no 11-12, 777-782 p.Article in journal (Refereed) Published
Abstract [en]

In this paper, a novel method is described for automated determination of dextromethorphan in biological fluids using molecularly imprinted solid-phase extraction (MISPE) as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and dextromethorphan as template molecule. These imprinted polymers were used as solid-phase extraction sorbent for the extraction of dextromethorphan from human plasma samples. Various parameters affecting the extraction efficiency of the MIP cartridges were evaluated. The high selectivity of the sorbent coupled to the high performance liquid chromatographic system permitted a simple and rapid analysis of this drug in plasma samples with limits of detection (LOD) and quantification (LOQ) of 0.12 ng/mL and 0.35 ng/mL, respectively. The MIP selectivity was evaluated by analyzing of the dextromethorphan in presence of several substances with similar molecular structures and properties. Results from the HPLC analyses showed that the recoveries of dextromethorphan using MIP cartridges from human plasma samples in the range of 1–50 ng/mL were higher than 87%.

Keyword
Molecularly imprinted polymer, Dextromethorphan, Human plasma, Automated, Sample clean-up
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-103346 (URN)10.1016/j.jchromb.2011.02.031 (DOI)
Available from: 2014-05-12 Created: 2014-05-12 Last updated: 2017-12-05Bibliographically approved
2. Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of human insulin in plasma and pharmaceutical formulations
Open this publication in new window or tab >>Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of human insulin in plasma and pharmaceutical formulations
2014 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 121, 30-36 p.Article in journal (Refereed) Published
Abstract [en]

In this paper, a novel method is described for automated determination of human insulin in biological fluids using principle of sequential injection on a molecularly imprinted solid-phase extraction (MISPE) cartridge as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, chloroform as a porogen and insulin as a template molecule. The imprinted polymers were then employed as the solid-phase extraction sorbent for on-line extraction of insulin from human plasma samples. To achieve the best condition, influential parameters on the extraction efficiency were thoroughly investigated. Rapid and simple analysis of the hormone was successfully accomplished through the good selectivity of the prepared sorbent coupled with HPLC. Limits of detection (LOD) and quantification (LOQ) of 0.2 ng mL−1, 0.7 ng mL−1, and 0.03 ng mL−1, 0.1 ng mL−1 were obtained in plasma and urine respectively. The obtained data exhibited the great recoveries for extraction of insulin from human plasma and pharmaceutical samples, higher than 87%.

Keyword
Molecularly imprinted polymer, Insulin, Human plasma, Pharmaceutical formulations, Automated, Sample clean-up
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-103355 (URN)10.1016/j.talanta.2013.12.011 (DOI)
Available from: 2014-05-12 Created: 2014-05-12 Last updated: 2017-12-05Bibliographically approved
3. On-line clean-up and determination of tramadol in human plasma and urine samples using molecularly imprinted monolithic column coupling with HPLC
Open this publication in new window or tab >>On-line clean-up and determination of tramadol in human plasma and urine samples using molecularly imprinted monolithic column coupling with HPLC
2012 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 911, no 12, 49-54 p.Article in journal (Refereed) Published
Abstract [en]

The applicability of an on-line solid phase extraction method using molecularly imprinted monolithic column was developed for the assay of tramadol (TRD) in urine and plasma samples. The monolithic column was prepared by using TRD as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker and chloroform as the porogen with in situ molecular imprinting polymerization technique. Various parameters affecting the extraction efficiency of the monolithic column were evaluated. Chromatographic analysis of TRD after on-line clean-up of samples was performed by reversed-phase HPLC on an ACE column with ultraviolet detection at 218 nm. The present work was successfully applied for automated simple analysis of TRD in urine and plasma samples with high recoveries between 90.5–93.1% and 93.3–96.0%, respectively. The results revealed that in concentration up to 500 ng/mL of dextromethorphan (DEX), timolol (TMO) and O-desmethyltramadol (M1), the recoveries were not reduced more than 4.3% and 4.0% for plasma and urine samples, respectively. The limit of detection (S/N = 3) and limit of quantification (S/N = 10) for TRD in urine samples were 0.03 ng/mL and 0.10 ng/mL, and in plasma samples were 0.3 and 1.0 ng/mL, respectively. Inter-column precision of the assays (n = 3) for urine and plasma samples at the 100 ng/mL TRD level were 4.0% and 4.2%, respectively.

Keyword
Molecularly imprinted polymer, Tramadol, Human Plasma, Monolithic column, Sample clean-up
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-103356 (URN)10.1016/j.jchromb.2012.10.019 (DOI)
Available from: 2014-05-12 Created: 2014-05-12 Last updated: 2017-12-05Bibliographically approved
4. Determination of Sarcosine in Plasma and Urine Samples Utilizing Dummy Molecularly Imprinted Polymer and Microextraction by Packed Sorbent on-line with Liquid Chromatography Tandem Mass Spectrometry
Open this publication in new window or tab >>Determination of Sarcosine in Plasma and Urine Samples Utilizing Dummy Molecularly Imprinted Polymer and Microextraction by Packed Sorbent on-line with Liquid Chromatography Tandem Mass Spectrometry
(English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573Article in journal (Refereed) Submitted
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-103361 (URN)
Available from: 2014-05-12 Created: 2014-05-12 Last updated: 2017-12-05Bibliographically approved
5. A novel needle trap utilizing molecularly imprinted-sol-gel xerogel for on-line microextraction of lung cancer biomarker bilirubin from plasma and urine samples
Open this publication in new window or tab >>A novel needle trap utilizing molecularly imprinted-sol-gel xerogel for on-line microextraction of lung cancer biomarker bilirubin from plasma and urine samples
(English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778Article in journal (Refereed) Submitted
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-103384 (URN)
Available from: 2014-05-13 Created: 2014-05-13 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

fulltext(1949 kB)653 downloads
File information
File name FULLTEXT02.pdfFile size 1949 kBChecksum SHA-512
52d1db203404f55df4e90eb0f0bdf6e0415c8594df94e3569e608cd4d835530e810e91cc295a36d551b9c6443a40d6285219e228efdebfecaa42a0fd3bf159d0
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Moein, Mohammad Mahdi
By organisation
Department of Analytical Chemistry
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 653 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 2000 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf