Change search
ReferencesLink to record
Permanent link

Direct link
Fructose bisphosphate aldolase is involved in the control of RNA polymerase III-directed transcription
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Show others and affiliations
2014 (English)In: Biochimica et Biophysica Acta. Molecular Cell Research, ISSN 0167-4889, Vol. 1843, no 6, 1103-1110 p.Article in journal (Refereed) Published
Abstract [en]

Yeast Fba1 (fructose 1,6-bisphosphate aldolase) is a glycolytic enzyme essential for viability. The overproduction of Fba1 enables overcoming of a severe growth defect caused by a missense mutation rpc128-1007 in a gene encoding the 028 protein, the second largest subunit of the RNA polymerase III complex. The suppression of the growth phenotype by Fbal is accompanied by enhanced de novo tRNA transcription in rpc128-1007 cells. We inactivated residues critical for the catalytic activity of Fbal. Overproduction of inactive aldolase still suppressed the rpc128-1007 phenotype, indicating that the function of this glycolytic enzyme in RNA polymerase III transcription is independent of its catalytic activity. Yeast Fbal was determined to interact with the RNA polymerase III complex by coimmunoprecipitation. Additionally, a role of aldolase in control of tRNA transcription was confirmed by ChIP experiments. The results indicate a novel direct relationship between RNA polymerase HI transcription and aldolase.

Place, publisher, year, edition, pages
2014. Vol. 1843, no 6, 1103-1110 p.
Keyword [en]
Aldolase, tRNA transcription, Pol III, Yeast
National Category
Biological Sciences
URN: urn:nbn:se:su:diva-104382DOI: 10.1016/j.bbamcr.2014.02.007ISI: 000335627900006OAI: diva2:723615


Available from: 2014-06-11 Created: 2014-06-10 Last updated: 2014-06-11Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Östlund Farrants, Ann-Kristin
By organisation
Department of Molecular Biosciences, The Wenner-Gren Institute
In the same journal
Biochimica et Biophysica Acta. Molecular Cell Research
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 169 hits
ReferencesLink to record
Permanent link

Direct link