Fructose bisphosphate aldolase is involved in the control of RNA polymerase III-directed transcription
2014 (English)In: Biochimica et Biophysica Acta. Molecular Cell Research, ISSN 0167-4889, Vol. 1843, no 6, 1103-1110 p.Article in journal (Refereed) Published
Yeast Fba1 (fructose 1,6-bisphosphate aldolase) is a glycolytic enzyme essential for viability. The overproduction of Fba1 enables overcoming of a severe growth defect caused by a missense mutation rpc128-1007 in a gene encoding the 028 protein, the second largest subunit of the RNA polymerase III complex. The suppression of the growth phenotype by Fbal is accompanied by enhanced de novo tRNA transcription in rpc128-1007 cells. We inactivated residues critical for the catalytic activity of Fbal. Overproduction of inactive aldolase still suppressed the rpc128-1007 phenotype, indicating that the function of this glycolytic enzyme in RNA polymerase III transcription is independent of its catalytic activity. Yeast Fbal was determined to interact with the RNA polymerase III complex by coimmunoprecipitation. Additionally, a role of aldolase in control of tRNA transcription was confirmed by ChIP experiments. The results indicate a novel direct relationship between RNA polymerase HI transcription and aldolase.
Place, publisher, year, edition, pages
2014. Vol. 1843, no 6, 1103-1110 p.
Aldolase, tRNA transcription, Pol III, Yeast
IdentifiersURN: urn:nbn:se:su:diva-104382DOI: 10.1016/j.bbamcr.2014.02.007ISI: 000335627900006OAI: oai:DiVA.org:su-104382DiVA: diva2:723615