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Solid-state NMR studies of metal-free SOD1 fibrillar structures
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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2014 (English)In: Journal of Biological Inorganic Chemistry, ISSN 0949-8257, E-ISSN 1432-1327, Vol. 19, no 4-5, 659-666 p.Article in journal (Refereed) Published
Abstract [en]

Copper-zinc superoxide dismutase 1 (SOD1) is present in the protein aggregates deposited in motor neurons of amyotrophic lateral sclerosis (ALS) patients. ALS is a neurodegenerative disease that can be either sporadic (ca. 90 %) or familial (fALS). The most widely studied forms of fALS are caused by mutations in the sequence of SOD1. Ex mortuo SOD1 aggregates are usually found to be amorphous. In vitro SOD1, in its immature reduced and apo state, forms fibrillar aggregates. Previous literature data have suggested that a monomeric SOD1 construct, lacking loops IV and VII, (apoSOD Delta IV-VII), shares the same fibrillization properties of apoSOD1, both proteins having the common structural feature of the central beta-barrel. In this work, we show that structural information can be obtained at a site-specific level from solid-state NMR. The residues that are sequentially assignable are found to be located at the putative nucleation site for fibrillar species formation in apoSOD, as detected by other experimental techniques.

Place, publisher, year, edition, pages
2014. Vol. 19, no 4-5, 659-666 p.
Keyword [en]
Copper-zinc superoxide dismutase 1, SOD1, Solid-state NMR, Fibrils, Aggregation, ALS
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:su:diva-105412DOI: 10.1007/s00775-014-1130-9ISI: 000336310000016OAI: diva2:729021


Available from: 2014-06-25 Created: 2014-06-24 Last updated: 2014-06-25Bibliographically approved

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Danielsson, JensLang, LisaOliveberg, Mikael
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Department of Biochemistry and Biophysics
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