Weak pulling forces exerted on N-in-orientated transmembrane segments during co-translational insertion into the inner membrane of Escherichia coli
2014 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 588, no 10, 1930-1934 p.Article in journal (Refereed) Published
Transmembrane helices (TMHs) in membrane proteins can be orientated with their N-terminus towards the cytoplasm (N-in), or facing the non-cytoplasmic side (N-out). Most membrane proteins are inserted co-translationally into membranes, aided by Sec-type translocons. Since the final orientation of N-in-and N-out-orientated TMHs differs, they could also interact differently with the translocon and the surrounding membrane during insertion. We measured pulling forces exerted on N-in-orientated TMHs during co-translational insertion into the inner membrane of Escherichia coli. Our results demonstrate that Nin-orientated TMHs experience a weaker pulling force but retain the overall biphasic force profile seen previously for Nout-orientated TMHs (Ismail et al., 2012 ).
Place, publisher, year, edition, pages
2014. Vol. 588, no 10, 1930-1934 p.
Membrane insertion, Transmembrane helix, Type 2 membrane protein
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-105193DOI: 10.1016/j.febslet.2014.03.050ISI: 000336033700011OAI: oai:DiVA.org:su-105193DiVA: diva2:732500
FunderEU, European Research Council, 232648Knut and Alice Wallenberg FoundationMistra - The Swedish Foundation for Strategic Environmental ResearchSwedish Research CouncilSwedish Cancer Society