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Weak pulling forces exerted on N-in-orientated transmembrane segments during co-translational insertion into the inner membrane of Escherichia coli
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
2014 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 588, no 10, 1930-1934 p.Article in journal (Refereed) Published
Abstract [en]

Transmembrane helices (TMHs) in membrane proteins can be orientated with their N-terminus towards the cytoplasm (N-in), or facing the non-cytoplasmic side (N-out). Most membrane proteins are inserted co-translationally into membranes, aided by Sec-type translocons. Since the final orientation of N-in-and N-out-orientated TMHs differs, they could also interact differently with the translocon and the surrounding membrane during insertion. We measured pulling forces exerted on N-in-orientated TMHs during co-translational insertion into the inner membrane of Escherichia coli. Our results demonstrate that Nin-orientated TMHs experience a weaker pulling force but retain the overall biphasic force profile seen previously for Nout-orientated TMHs (Ismail et al., 2012 [1]).

Place, publisher, year, edition, pages
2014. Vol. 588, no 10, 1930-1934 p.
Keyword [en]
Membrane insertion, Transmembrane helix, Type 2 membrane protein
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-105193DOI: 10.1016/j.febslet.2014.03.050ISI: 000336033700011OAI: oai:DiVA.org:su-105193DiVA: diva2:732500
Funder
EU, European Research Council, 232648Knut and Alice Wallenberg FoundationMistra - The Swedish Foundation for Strategic Environmental ResearchSwedish Research CouncilSwedish Cancer Society
Note

AuthorCount:3;

Available from: 2014-07-04 Created: 2014-06-24 Last updated: 2017-12-05Bibliographically approved

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Cymer, FlorianIsmail, Nurzianvon Heijne, Gunnar
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