Secondary stimulation from Bacillus Calmette-Guerin induced macrophages induce nitric oxide independent cell-death in bladder cancer cells
2014 (English)In: Cancer Letters, ISSN 0304-3835, E-ISSN 1872-7980, Vol. 348, no 1-2, 119-125 p.Article in journal (Refereed) Published
The anti-tumour mechanisms following Bacillus Calmette-Guerin (BCG) treatment of bladder-cancer remain largely unknown. Previous studies have shown involvement of nitric-oxide (NO) formation in the BCG-mediated effect. We analyzed the effects of macrophage secreted factors (MSFs) from BCG-stimulated RAW264.7 cells on the bladder-cancer cell line MBT2. Direct treatment with BCG did not induce NO in MBT2-cells whereas supernatant from BCG-stimulated macrophages increased NOS2 mRNA and protein expression, NO concentrations and cell-death. Blocking NO-synthesis with the NOS-inhibitor L-NAME did not affect levels of cell-death suggesting cytotoxic pathways involving other signalling molecules than NO. Several such candidate genes were identified in a microarray.
Place, publisher, year, edition, pages
2014. Vol. 348, no 1-2, 119-125 p.
Urinary bladder cancer, Bacillus Calmette-Guerin, Nitric oxide, Nitric oxide synthase, Cytokines
Cancer and Oncology Microbiology
IdentifiersURN: urn:nbn:se:su:diva-105885DOI: 10.1016/j.canlet.2014.03.014ISI: 000336878100013OAI: oai:DiVA.org:su-105885DiVA: diva2:733417