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Identification of novel sphingolipid-binding motifs in mammalian membrane proteins
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
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2014 (English)In: Biochimica et Biophysica Acta - Biomembranes, ISSN 0005-2736, E-ISSN 1879-2642, Vol. 1838, no 8, 2066-2070 p.Article in journal (Refereed) Published
Abstract [en]

Specific interactions between transmembrane proteins and sphingolipids is a poorly understood phenomenon, and only a couple of instances have been identified. The best characterized example is the sphingolipid-binding motif VXXTLXXIY found in the transmembrane helix of the vesicular transport protein p24. Here, we have used a simple motif-probability algorithm (MOPRO) to identify proteins that contain putative sphingolipid-binding motifs in a dataset comprising proteomes from mammalian organisms. From these motif-containing candidate proteins, four with different numbers of transmembrane helices were selected for experimental study: i) major histocompatibility complex II Q alpha chain subtype (DQA1), ii) GPI-attachment protein 1 (GAA1), iii) tetraspanin-7 TSN7, and iv), metabotropic glutamate receptor 2 (GRM2). These candidates were subjected to photo-affinity labeling using radiolabeled sphingolipids, confirming all four candidate proteins as sphingolipid-binding proteins. The sphingolipid-binding motifs are enriched in the 7TM family of G-protein coupled receptors, predominantly in transmembrane helix 6. The ability of the motif-containing candidate proteins to bind sphingolipids with high specificity opens new perspectives on their respective regulation and function.

Place, publisher, year, edition, pages
2014. Vol. 1838, no 8, 2066-2070 p.
Keyword [en]
Sphingolipid, Transmembrane helix, Membrane protein
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:su:diva-106312DOI: 10.1016/j.bbamem.2014.04.026ISI: 000337984900008OAI: diva2:736150


Available from: 2014-08-05 Created: 2014-08-04 Last updated: 2014-08-05Bibliographically approved

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Björkholm, Patrikvon Heijne, Gunnar
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Department of Biochemistry and BiophysicsScience for Life Laboratory (SciLifeLab)
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