Application of Qualitative and Quantitative Real-Time PCR, Direct Sequencing, and Terminal Restriction Fragment Length Polymorphism Analysis for Detection and Identification of Polymicrobial 16S rRNA Genes in Ascites
Number of Authors: 10
2014 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 52, no 5, 1754-1757 p.Article in journal (Refereed) Published
Qualitative and quantitative 16S rRNA gene-based real-time PCR and direct sequencing were applied for rapid detection and identification of bacterial DNA (bactDNA) in 356 ascites samples. bactDNA was detected in 35% of samples, with a mean of 3.24 log copies ml(-1). Direct sequencing of PCR products revealed 62% mixed chromatograms predominantly belonging to Grampositive bacteria. Terminal restriction fragment length polymorphism (T-RFLP) results of a sample subset confirmed sequence data showing polymicrobial DNA contents in 67% of bactDNA-positive ascites samples.
Place, publisher, year, edition, pages
2014. Vol. 52, no 5, 1754-1757 p.
IdentifiersURN: urn:nbn:se:su:diva-106445DOI: 10.1128/JCM.00552-14ISI: 000337915700072OAI: oai:DiVA.org:su-106445DiVA: diva2:736198