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Application of Qualitative and Quantitative Real-Time PCR, Direct Sequencing, and Terminal Restriction Fragment Length Polymorphism Analysis for Detection and Identification of Polymicrobial 16S rRNA Genes in Ascites
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Number of Authors: 10
2014 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 52, no 5, 1754-1757 p.Article in journal (Refereed) Published
Abstract [en]

Qualitative and quantitative 16S rRNA gene-based real-time PCR and direct sequencing were applied for rapid detection and identification of bacterial DNA (bactDNA) in 356 ascites samples. bactDNA was detected in 35% of samples, with a mean of 3.24 log copies ml(-1). Direct sequencing of PCR products revealed 62% mixed chromatograms predominantly belonging to Grampositive bacteria. Terminal restriction fragment length polymorphism (T-RFLP) results of a sample subset confirmed sequence data showing polymicrobial DNA contents in 67% of bactDNA-positive ascites samples.

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2014. Vol. 52, no 5, 1754-1757 p.
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Microbiology
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URN: urn:nbn:se:su:diva-106445DOI: 10.1128/JCM.00552-14ISI: 000337915700072OAI: oai:DiVA.org:su-106445DiVA: diva2:736198
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AuthorCount:10;

Available from: 2014-08-05 Created: 2014-08-04 Last updated: 2017-12-05Bibliographically approved

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