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Fusion of Protein Aggregates Facilitates Asymmetric Damage Segregation
Stockholm University, Faculty of Science, Stockholm Resilience Centre. Max Planck Institute for the Physics of Complex Systems, Germany.
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Number of Authors: 5
2014 (English)In: PLoS biology, ISSN 1544-9173, E-ISSN 1545-7885, Vol. 12, no 6, e1001886- p.Article in journal (Refereed) Published
Abstract [en]

Asymmetric segregation of damaged proteins at cell division generates a cell that retains damage and a clean cell that supports population survival. In cells that divide asymmetrically, such as Saccharomyces cerevisiae, segregation of damaged proteins is achieved by retention and active transport. We have previously shown that in the symmetrically dividing Schizosaccharomyces pombe there is a transition between symmetric and asymmetric segregation of damaged proteins. Yet how this transition and generation of damage-free cells are achieved remained unknown. Here, by combining in vivo imaging of Hsp104-associated aggregates, a form of damage, with mathematical modeling, we find that fusion of protein aggregates facilitates asymmetric segregation. Our model predicts that, after stress, the increased number of aggregates fuse into a single large unit, which is inherited asymmetrically by one daughter cell, whereas the other one is born clean. We experimentally confirmed that fusion increases segregation asymmetry, for a range of stresses, and identified Hsp16 as a fusion factor. Our work shows that fusion of protein aggregates promotes the formation of damage-free cells. Fusion of cellular factors may represent a general mechanism for their asymmetric segregation at division.

Place, publisher, year, edition, pages
2014. Vol. 12, no 6, e1001886- p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-106435DOI: 10.1371/journal.pbio.1001886ISI: 000337972900013OAI: oai:DiVA.org:su-106435DiVA: diva2:736257
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AuthorCount:5;

Available from: 2014-08-05 Created: 2014-08-04 Last updated: 2017-12-05Bibliographically approved

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