Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes?
Number of Authors: 13
2014 (English)In: Radiation Protection Dosimetry, ISSN 0144-8420, E-ISSN 1742-3406, Vol. 159, no 1-4, 87-94 p.Article in journal (Refereed) Published
Within the EU MULTIBIODOSE project, the automated micronucleus (MN) assay was optimised for population triage in large-scale radiological emergencies. For MN scoring, two approaches were applied using the Metafer4 platform (MetaSystems, Germany): fully automated scoring and semi-automated scoring with visual inspection of the gallery of MN-positive objects. Dose-response curves were established for acute and protracted whole-body and partial-body exposures. A database of background MN yields was set up, allowing determination of the dose detection threshold in both scoring modes. An analysis of the overdispersion of the MN frequency distribution sigma(2)/A mu obtained by semi-automated scoring showed that the value of this parameter represents a reliability check of the calculated equivalent total body dose in case the accident overexposure is a partial-body exposure. The elaborated methodology was validated in an accident training exercise. Overall, the semi-automated scoring procedure represents important added value to the automated MN assay.
Place, publisher, year, edition, pages
2014. Vol. 159, no 1-4, 87-94 p.
Earth and Related Environmental Sciences
IdentifiersURN: urn:nbn:se:su:diva-106606DOI: 10.1093/rpd/ncu130ISI: 000338829100012OAI: oai:DiVA.org:su-106606DiVA: diva2:737624