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Macrophage Control of Phagocytosed Mycobacteria Is Increased by Factors Secreted by Alveolar Epithelial Cells through Nitric Oxide Independent Mechanisms
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
2014 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, no 8, e103411- p.Article in journal (Refereed) Published
Abstract [en]

Tissue-resident macrophages are heterogeneous with tissue-specific and niche-specific functions. Thus, simplified models of macrophage activation do not explain the extent of heterogeneity seen in vivo. We focus here on the respiratory tract and ask whether factors secreted by alveolar epithelial cells (AEC) can influence the functionality of resident pulmonary macrophages (PuM). We have previously reported that factors secreted by AEC increase control of intracellular growth of BCG in macrophages. In the current study, we also aimed to investigate possible mechanisms by which AEC-derived factors increase intracellular control of BCG in both primary murine interstitial macrophages, and bone marrow-derived macrophages and characterize further the effect of these factors on macrophage differentiation. We show that; a) in contrast to other macrophage types, IFN-gamma did not increase intracellular growth control of Mycobacterium bovis, Bacillus Calmette-Guerin (BCG) by interstitial pulmonary macrophages although the same macrophages could be activated by factors secreted by AEC; b) the lack of response of pulmonary macrophages to IFN-gamma was apparently regulated by suppressor of cytokine signaling (SOCS) 1; c) AEC-derived factors did not induce pro-inflammatory pathways induced by IFN-gamma e.g. expression of inducible nitric oxide synthase (iNOS), secretion of nitric oxide (NO), or IL-12, d) in contrast to IFN-gamma, intracellular bacterial destruction induced by AEC-derived factors was not dependent on iNOS transcription and NO production. Collectively, our data show that PuM were restricted in inflammatory responses mediated by IFN-gamma through SOCS1 and that factors secreted by AEC-enhanced the microbicidal capacities of macrophages by iNOS independent mechanisms.

Place, publisher, year, edition, pages
2014. Vol. 9, no 8, e103411- p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-107114DOI: 10.1371/journal.pone.0103411ISI: 000339812700023OAI: oai:DiVA.org:su-107114DiVA: diva2:743337
Note

AuthorCount:4;

Available from: 2014-09-03 Created: 2014-09-03 Last updated: 2017-12-05Bibliographically approved

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