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Assembly of nonheme Mn/Fe active sites in heterodinuclear metalloproteins
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
2014 (English)In: Journal of Biological Inorganic Chemistry, ISSN 0949-8257, E-ISSN 1432-1327, Vol. 19, no 6, 759-774 p.Article, review/survey (Refereed) Published
Abstract [en]

The ferritin superfamily contains several protein groups that share a common fold and metal coordinating ligands. The different groups utilize different dinuclear cofactors to perform a diverse set of reactions. Several groups use an oxygen-activating di-iron cluster, while others use di-manganese or heterodinuclear Mn/Fe cofactors. Given the similar primary ligand preferences of Mn and Fe as well as the similarities between the binding sites, the basis for metal specificity in these systems remains enigmatic. Recent data for the heterodinuclear cluster show that the protein scaffold per se is capable of discriminating between Mn and Fe and can assemble the Mn/Fe center in the absence of any potential assembly machineries or metal chaperones. Here we review the current understanding of the assembly of the heterodinuclear cofactor in the two different protein groups in which it has been identified, ribonucleotide reductase R2c proteins and R2-like ligand-binding oxidases. Interestingly, although the two groups form the same metal cluster they appear to employ partly different mechanisms to assemble it. In addition, it seems that both the thermodynamics of metal binding and the kinetics of oxygen activation play a role in achieving metal specificity.

Place, publisher, year, edition, pages
2014. Vol. 19, no 6, 759-774 p.
Keyword [en]
Affinity, Chlamydia, Cofactor maturation, Metal selection, Specificity
National Category
Biological Sciences Chemical Sciences
URN: urn:nbn:se:su:diva-107025DOI: 10.1007/s00775-014-1140-7ISI: 000339975100005OAI: diva2:743618


Available from: 2014-09-04 Created: 2014-09-02 Last updated: 2014-09-04Bibliographically approved

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Griese, Julia J.Srinivas, VivekHögbom, Martin
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