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Medium from gamma-irradiated Escherichia coli bacteria stimulates a unique immune response in Drosophila cells
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
2014 (English)In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 46, no 2, 392-400 p.Article in journal (Refereed) Published
Abstract [en]

It is well known that gamma-irradiated, non-dividing bacteria can elicit potent immune responses in mammals. Compared to traditional heat or chemical inactivation of microbes, gamma -irradiation likely preserves metabolic activity and antigenic features to a larger extent. We have previously shown that antimicrobial peptides are induced in Drosophila by peptidoglycan fragments secreted into the medium of exponentially growing bacterial cultures. In this study, we gamma-irradiated Escherichia coil cells at a dose that halted cell division. The temporal synthesis and release of peptidoglycan fragments were followed as well as the potential of bacterial supernatants to induce immune responses in Drosophila S2 cells. We demonstrate that peptidoglycan synthesis continues for several days post irradiation and that monomeric peptidoglycan is shed into the medium. Whole transcriptome analysis revealed a strong immune response against the bacterial medium. The response to medium taken directly post irradiation shows a large overlap to that of peptidoglycan. Medium from prolonged bacterial incubation does, however, stimulate a selective set of immune genes. A shift towards a stress response was instead observed with a striking induction of several heat shock proteins. Our findings suggest that gamma-irradiated bacteria release elicitors that stimulate a novel response in Drosophila.

Place, publisher, year, edition, pages
2014. Vol. 46, no 2, 392-400 p.
Keyword [en]
Peptidoglycan, S2 cells, Antimicrobial peptides, Heat shock proteins
National Category
Zoology Immunology
Identifiers
URN: urn:nbn:se:su:diva-107084DOI: 10.1016/j.dci.2014.05.018ISI: 000340216600030OAI: oai:DiVA.org:su-107084DiVA: diva2:744224
Note

AuthorCount:3;

Available from: 2014-09-07 Created: 2014-09-03 Last updated: 2017-12-05Bibliographically approved

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Lindberg, Bo G.Steiner, Håkan
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