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A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification
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2014 (English)In: BioTechniques, ISSN 0736-6205, E-ISSN 1940-9818, Vol. 57, no 2, 81-87 p.Article in journal (Refereed) Published
Abstract [en]

The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used. We have compared the performance of conventional Taq and Bst DNA polymerases to a novel Tag DNA polymerase mutant (SD DNA polymerase), which has a strong strand displacement activity, in PCR (including amplification of GC-rich and complex secondary structure templates), long-range PCR (LR PCR), loop-mediated amplification (LAMP), and polymerase chain displacement reaction (PCDR). Our results demonstrate that the strand displacement activity of SD DNA polymerase, in combination with the robust polymerase activity, provides a notable improvement in the sensitivity and efficiency of all these methods.

Place, publisher, year, edition, pages
2014. Vol. 57, no 2, 81-87 p.
Keyword [en]
PCR, PCDR, LAMP, strand displacement, DNA polymerase, quantitative amplification, real-time amplification, isothermal amplification
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-107430DOI: 10.2144/000114198ISI: 000340582000005OAI: oai:DiVA.org:su-107430DiVA: diva2:748395
Note

AuthorCount:6;

Available from: 2014-09-19 Created: 2014-09-15 Last updated: 2017-12-05Bibliographically approved

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Kramarova, Tatiana V.
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Department of Molecular Biosciences, The Wenner-Gren Institute
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