Organization of mitochondrial gene expression in two distinctribosome-containing assemblies
(English)Manuscript (preprint) (Other academic)
Mitochondria contain their own genetic system that provides subunits of the complexesdriving oxidative phosphorylation. A quarter of the mitochondrial proteome participates ingene expression, but how all these factors are orchestrated and spatially organized is currentlyunknown. Here we developed a novel method to purify native complexes of mitochondrialribosomes. Quantitative mass-spectrometry revealed extensive interactions of ribosomes withfactors that represent all the steps of post-transcriptional gene expression. These interactionsresult in large expressosomes-like assemblies that we termed MIOREX (MItochondrialORganization of gene EXpression) complexes. Most MIOREX complexes are evenlydistributed throughout the mitochondrial network, while a subset is present as nucleoid-MIOREX complexes that unite the whole spectrum of organellar gene expression. Our worktherefore provides a novel conceptual framework for the spatial organization of mitochondrialprotein synthesis that likely developed to facilitate gene expression in the organelle.
Biochemistry and Molecular Biology
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:su:diva-107645OAI: oai:DiVA.org:su-107645DiVA: diva2:748890