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On-Chip Detection of Rolling Circle Amplified DNA Molecules from Bacillus Globigii Spores and Vibrio Cholerae
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2014 (English)In: Small, ISSN 1613-6810, E-ISSN 1613-6829, Vol. 10, no 14, 2877-2882 p.Article in journal (Refereed) Published
Abstract [en]

For the first time DNA coils formed by rolling circle amplification are quantified on-chip by Brownian relaxation measurements on magnetic nanobeads using a magnetoresistive sensor. No external magnetic fields are required besides the magnetic field arising from the current through the sensor, which makes the setup very compact. Limits of detection down to 500 Bacillus globigii spores and 2 pM of Vibrio cholerae are demonstrated, which are on the same order of magnitude or lower than those achieved previously using a commercial macro-scale AC susceptometer. The chip-based readout is an important step towards the realization of field tests based on rolling circle amplification molecular analyses.

Place, publisher, year, edition, pages
2014. Vol. 10, no 14, 2877-2882 p.
National Category
Biological Sciences
URN: urn:nbn:se:su:diva-107631DOI: 10.1002/smll.201303325ISI: 000340660900019OAI: diva2:748959


Available from: 2014-09-22 Created: 2014-09-22 Last updated: 2014-09-22Bibliographically approved

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Mezger, AnjaNilsson, Mats
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Department of Biochemistry and BiophysicsScience for Life Laboratory (SciLifeLab)
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