Insertion of marginally hydrophobic helix in EmrD
2014 (English)Manuscript (preprint) (Other academic)
The positive inside rule governs the orientation of membrane proteins in such a way that more positively chargedamino acids are found on the inside of a cell. The exact mechanisms of how this is achieved are not well known, butit is clear that positively charged residues can facilitate the insertion of transmembrane helices that are not sufficientlyhydrophobic to be inserted otherwise. Here, we study one such helix, helix 2 in EmrD. We show that the insertionof this helix is facilitated when followed by positively charged residues and a hydrophobic helix. Surprisingly thepositively charged residues are not sufficient alone. This further strengthens our earlier observations that the last helixneeds to be more hydrophobic than previous helices.
Place, publisher, year, edition, pages
Biochemistry and Molecular Biology
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:su:diva-108912OAI: oai:DiVA.org:su-108912DiVA: diva2:763802