Linking sub-cellular biomarkers to embryo aberrations in the benthic amphipod Monoporeia affinis
(English)Manuscript (preprint) (Other academic)
To adequately assess and monitor environmental status in the aquatic environment a broad approach is needed that integrates physical variables, chemical analyses and biological effects at different levels of the biological organization. Embryo aberrations in the Baltic Sea key species Monoporeia affinis have shown to be inducible by both metals and organic substances as well as by hypoxia, increasing temperatures and malnutrition. This amphipod have therefore been used for more than three decades as a biological effect indicator in monitoring and assessment of chemical pollution and environmental stress. However, little is known about the sub-cellular mechanisms underlying embryo aberrations. An improved mechanistic understanding may open up the possibility of including sub-cellular alterations as sensitive warning signals of stress-induced embryo aberrations. In the present study, M. affinis was exposed in microcosms to 4 different sediments from the Baltic Sea. After 88-95 days of exposure, survival and fecundity was determined as well as the frequency and type of embryo aberrations. Moreover, oxygen radical absorption capacity (ORAC) was assayed as a proxy for antioxidant defence, thiobarbituric acid reactive substances (TBARS) as a measurement of lipid peroxidation and acetylcholinesterase (AChE) activity as an indicator of neurotoxicity. The results show that AChE and ORAC can be linked to the frequency of malformed embryos and arrested embryo development. The occurrence of dead broods was significantly associated with elevated TBARS levels. It can be concluded that these sub-cellular biomarkers are indicative of effects that could affect Darwinian fitness and that oxidative stress is a likely mechanism in the development of aberrant embryos.
Monoporeia affinis, embryo aberrations, oxidative stress, Darwinian fitness
Research subject Applied Environmental Science
IdentifiersURN: urn:nbn:se:su:diva-111065OAI: oai:DiVA.org:su-111065DiVA: diva2:774043