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Labelling of N-hydroxysuccinimide-modified rhodamine B on cellulose nanofibrils by the amidation reaction
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
2014 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 4, no 105, 60757-60761 p.Article in journal (Refereed) Published
Abstract [en]

We have labelled cellulose nanofibrils with rhodamine B and characterized the grafting and the fluorescent properties by infrared and UV/visible spectroscopy together with fluorescence spectroscopy and confocal laser scanning microscopy. The cellulose nanofibrils were surface modified with amine groups using 4-(Boc-aminomethyl)phenyl isothiocyanate, that allows grafting of a wide number of commercially available molecules that e.g. contain carboxylic acid groups. The amine-modified cellulose nanofibrils were reacted with N-hydroxysuccinimide-modified rhodamine B ester through the amidation reaction. The labeling of the dye onto CNF results in a red-shift of the absorption and emission bands and despite some quenching of the fluorescence intensity of the surface-bound chromophore, it was possible to image the luminescent CNF at both low and high magnification using a confocal laser scanning microscope.

Place, publisher, year, edition, pages
2014. Vol. 4, no 105, 60757-60761 p.
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Chemical Sciences
Identifiers
URN: urn:nbn:se:su:diva-111709DOI: 10.1039/c4ra06559jISI: 000345655600041OAI: oai:DiVA.org:su-111709DiVA: diva2:776504
Note

AuthorCount:2;

Available from: 2015-01-07 Created: 2015-01-07 Last updated: 2017-12-05Bibliographically approved

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Navarro, Julien R. G.Bergström, Lennart
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