A single step reversed-phase high performance liquid chromatography separation of polar and non-polar lipids
2014 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1369, 105-115 p.Article in journal (Refereed) Published
This paper reports a simple chromatographic system to separate lipids classes as well as their molecular species. By the use of phenyl coated silica as stationary phase in combination with a simple mobile phase consisting of methanol and water, all tested lipid classes elute within 30min. Furthermore, a method to accurately predict retention times of specific lipid components for this type of chromatography is presented. Common detection systems were used, namely evaporative light scattering detection (ELSD), charged aerosol detection (CAD), electrospray mass spectrometry (ESI-MS), and UV detection.
Place, publisher, year, edition, pages
2014. Vol. 1369, 105-115 p.
Reversed phase liquid chromatography, Glycerolipids, Phospholipids, Evaporative light scattering detector, Charged aerosol detector, Mass spectrometry
IdentifiersURN: urn:nbn:se:su:diva-111915DOI: 10.1016/j.chroma.2014.10.010ISI: 000345489000013OAI: oai:DiVA.org:su-111915DiVA: diva2:778869