Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Revisiting cell specification and differentiation in the Drosophila airways, an insect organ homologous to our lung and blood vessels
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Oxygen is essential for life. Aquatic ancestral animal species are thought to have independently terrestrialized and invented distinct strategies for efficient oxygen supply. The respiratory system of vertebrates like us is composed of lungs connected to the vasculature while insects have a single system delivering air directly to internal tissues. In spite of their different evolutionary histories, the formation of these different tubular networks is thought to share many cellular, genetic and molecular principles. Here, in register with preceding studies, I briefly introduce the projects of my co-authors and me, addressing several new aspects of specification and differentiation of the Drosophila airways.

The airway primordia are specified at the lateral ectoderm of each side of the embryo as 10 groups of epithelial cells. These cells coordinately invaginate from the 2-dimensional (2D) ectodermal sheet to form 3D primitive tubes. The most proximal cells to the epidermis take the pluripotent cell fate and later generate most of the pupal and adult airways. Distal cells ramify to establish the primary branches and some neighboring branches fuse, interconnecting the network. Establishing these basic architectures, the tubular network matures into functional airways, attaining proper tube sizes in diameter and length, producing an annular-ridged lining of exoskeleton to avoid tube collapse and finally filling the system with gas.

First, I present airway-promoting functions of factors that were previously assigned to repress the airway fate. Then, I present genetic frameworks discriminating between 3 ground cell fates and the more derived cell fates: A) the proximal pluripotent cells vs. the distal more differentiated cells, B) the visceral branch vs. the signal-induced primary branches and C) the 1st metamere vs. the more posterior metameres. Lastly, I present our efforts to identify genes converting the airway tubes into a functional respiratory system.

Place, publisher, year, edition, pages
Stockholm: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University , 2015.
Keyword [en]
cell differentiation, airways, Drosophila, cell signaling, transcription factors
National Category
Developmental Biology
Research subject
Developmental Biology
Identifiers
URN: urn:nbn:se:su:diva-114174ISBN: 978-91-7649-106-5 (print)OAI: oai:DiVA.org:su-114174DiVA: diva2:790126
Public defence
2015-03-27, sal E306, Arrheniuslaboratorierna, Svante Arrhenius väg 20 C, Stockholm, 13:00 (English)
Opponent
Supervisors
Note

At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 1: Manuscript. Paper 2: Manuscript. Paper 3: Manuscript. Paper 5: Manuscript.

Available from: 2015-03-05 Created: 2015-02-23 Last updated: 2015-03-10Bibliographically approved
List of papers
1. Decapentaplegic/BMP and dEGFR promote the airway cell fate in Drosophila
Open this publication in new window or tab >>Decapentaplegic/BMP and dEGFR promote the airway cell fate in Drosophila
(English)Manuscript (preprint) (Other academic)
National Category
Developmental Biology
Research subject
Developmental Biology
Identifiers
urn:nbn:se:su:diva-114179 (URN)
Available from: 2015-02-23 Created: 2015-02-23 Last updated: 2016-01-29Bibliographically approved
2. A genetic basis for pluripotent versus differentiated cell fate selection during early development of the Drosophila airways
Open this publication in new window or tab >>A genetic basis for pluripotent versus differentiated cell fate selection during early development of the Drosophila airways
(English)Manuscript (preprint) (Other academic)
National Category
Developmental Biology
Research subject
Developmental Biology
Identifiers
urn:nbn:se:su:diva-114180 (URN)
Available from: 2015-02-23 Created: 2015-02-23 Last updated: 2016-01-29Bibliographically approved
3. Antagonistic interactions between the Emx ortholog Empty spiracle with Wingless/WNT, Dpp/BMP and Hox proteins induce branch-specific apoptotic pruning in the Drosophila airways
Open this publication in new window or tab >>Antagonistic interactions between the Emx ortholog Empty spiracle with Wingless/WNT, Dpp/BMP and Hox proteins induce branch-specific apoptotic pruning in the Drosophila airways
(English)Manuscript (preprint) (Other academic)
National Category
Developmental Biology
Research subject
Developmental Biology
Identifiers
urn:nbn:se:su:diva-114181 (URN)
Available from: 2015-02-23 Created: 2015-02-23 Last updated: 2016-01-29Bibliographically approved
4. The Intersection of the Extrinsic Hedgehog and WNT/Wingless Signals with the Intrinsic Hox Code Underpins Branching Pattern and Tube Shape Diversity in the Drosophila Airways
Open this publication in new window or tab >>The Intersection of the Extrinsic Hedgehog and WNT/Wingless Signals with the Intrinsic Hox Code Underpins Branching Pattern and Tube Shape Diversity in the Drosophila Airways
2015 (English)In: PLOS Genetics, ISSN 1553-7390, E-ISSN 1553-7404, Vol. 11, no 1, e1004929Article in journal (Refereed) Published
Abstract [en]

The tubular networks of the Drosophila respiratory system and our vasculature show distinct branching patterns and tube shapes in different body regions. These local variations are crucial for organ function and organismal fitness. Organotypic patterns and tube geometries in branched networks are typically controlled by variations of extrinsic signaling but the impact of intrinsic factors on branch patterns and shapes is not well explored. Here, we show that the intersection of extrinsic hedgehog(hh) and WNT/wingless (wg) signaling with the tube-intrinsic Hox code of distinct segments specifies the tube pattern and shape of the Drosophila airways. In the cephalic part of the airways, hh signaling induces expression of the transcription factor (TF) knirps (kni) in the anterior dorsal trunk (DTa1). kni represses the expression of another TF spalt major (salm), making DTa1 a narrow and long tube. In DTa branches of more posterior metameres, Bithorax Complex (BX-C) Hox genes autonomously divert hh signaling from inducing kni, thereby allowing DTa branches to develop as salm-dependent thick and short tubes. Moreover, the differential expression of BX-C genes is partly responsible for the anterior-to-posterior gradual increase of the DT tube diameter through regulating the expression level of Salm, a transcriptional target of WNT/wg signaling. Thus, our results highlight how tube intrinsic differential competence can diversify tube morphology without changing availabilities of extrinsic factors.

National Category
Developmental Biology
Research subject
Developmental Biology
Identifiers
urn:nbn:se:su:diva-114182 (URN)10.1371/journal.pgen.1004929 (DOI)000349314600041 ()
Available from: 2015-02-23 Created: 2015-02-23 Last updated: 2017-12-04Bibliographically approved
5. Transient junction anisotropies adjust 3-dimensional cell polarization to tissue geometry
Open this publication in new window or tab >>Transient junction anisotropies adjust 3-dimensional cell polarization to tissue geometry
(English)Manuscript (preprint) (Other academic)
National Category
Developmental Biology
Research subject
Developmental Biology
Identifiers
urn:nbn:se:su:diva-114183 (URN)
Available from: 2015-02-23 Created: 2015-02-23 Last updated: 2016-01-29Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Matsuda, Ryo
By organisation
Department of Molecular Biosciences, The Wenner-Gren Institute
Developmental Biology

Search outside of DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 298 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf