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In vitro generation of motor neuron precursors from mouse embryonic stem cells using mesoporous nanoparticles
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
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2014 (English)In: Nanomedicine, ISSN 1743-5889, E-ISSN 1748-6963, Vol. 9, no 16, 2457-2466 p.Article in journal (Refereed) Published
Abstract [en]

Aim: Stem cell-derived motor neurons (MNs) are utilized to develop replacement strategies for spinal cord disorders. Differentiation of embryonic stem cells into MN precursors involves factors and their repeated administration. We investigated if delivery of factors loaded into mesoporous nanoparticles could be effective for stem cell differentiation in vitro. Materials & methods: We used a mouse embryonic stem cell line expressing green fluorescent protein under the promoter for the MN-specific gene Hb9 to visualize the level of MN differentiation. The differentiation of stem cells was evaluated by expression of MN-specific transcription factors monitored by quantitative real-time PCR reactions and immunocytochemistry. Results: Mesoporous nanoparticles have strong affiliation to the embryoid bodies, penetrate inside the embryoid bodies and come in contact with differentiating cells. Conclusion: Repeated administration of soluble factors into a culture medium can be avoided due to a sustained release effect using mesoporous silica.

Place, publisher, year, edition, pages
2014. Vol. 9, no 16, 2457-2466 p.
Keyword [en]
cell transplant, differentiation, mesoporous material, nanomedicine, stem cell
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Nano Technology Chemical Sciences
Identifiers
URN: urn:nbn:se:su:diva-113143DOI: 10.2217/NNM.14.23ISI: 000346177600004OAI: oai:DiVA.org:su-113143DiVA: diva2:791339
Note

AuthorCount:9;

Available from: 2015-02-27 Created: 2015-01-23 Last updated: 2017-12-04Bibliographically approved

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Garcia-Bennett, Alfonso E.
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Nanomedicine
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)Nano TechnologyChemical Sciences

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