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Integration of body temperature into the analysis of energy expenditure in the mouse
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute. National Institute of Diabetes and Digestive and Kidney Diseases, NIH, USA.
2015 (English)In: Molecular Metabolism, ISSN 2212-8778, Vol. 4, no 6, 461-470 p.Article in journal (Refereed) Published
Abstract [en]

Objectives: We quantified the effect of environmental temperature on mouse energy homeostasis and body temperature.Methods: The effect of environmental temperature (4e33 C) on body temperature, energy expenditure, physical activity, and food intake invarious mice (chow diet, high-fat diet, Brs3-/y, lipodystrophic) was measured using continuous monitoring.Results: Body temperature depended most on circadian phase and physical activity, but also on environmental temperature. The amounts ofenergy expenditure due to basal metabolic rate (calculated via a novel method), thermic effect of food, physical activity, and cold-inducedthermogenesis were determined as a function of environmental temperature. The measured resting defended body temperature matchedthat calculated from the energy expenditure using Fourier’s law of heat conduction. Mice defended a higher body temperature during physicalactivity. The cost of the warmer body temperature during the active phase is 4e16% of total daily energy expenditure. Parameters measured indiet-induced obese and Brs3-/y mice were similar to controls. The high post-mortem heat conductance demonstrates that most insulation in miceis via physiological mechanisms.Conclusions: At 22 C, cold-induced thermogenesis isw120% of basal metabolic rate. The higher body temperature during physical activity isdue to a higher set point, not simply increased heat generation during exercise. Most insulation in mice is via physiological mechanisms, with littlefrom fur or fat. Our analysis suggests that the definition of the upper limit of the thermoneutral zone should be re-considered. Measuring bodytemperature informs interpretation of energy expenditure data and improves the predictiveness and utility of the mouse to model human energyhomeostasis.

Place, publisher, year, edition, pages
2015. Vol. 4, no 6, 461-470 p.
Keyword [en]
Thermoneutrality, Basal metabolic rate, Cold-induced thermogenesis, Body temperature, Energy expenditure, Heat conductance
National Category
Biochemistry and Molecular Biology
Research subject
Physiology
Identifiers
URN: urn:nbn:se:su:diva-115870DOI: 10.1016/j.molmet.2015.03.001ISI: 000367439600002OAI: oai:DiVA.org:su-115870DiVA: diva2:800513
Available from: 2015-04-06 Created: 2015-04-06 Last updated: 2016-02-01Bibliographically approved
In thesis
1. Thermal physiology and metabolism: Interplay between heat generation and energy homeostasis
Open this publication in new window or tab >>Thermal physiology and metabolism: Interplay between heat generation and energy homeostasis
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Mammal metabolism is intimately connected to the maintenance of body temperature. While metabolic pathways invariably produce heat as a by-product, the natural heat present in the environment also plays a role in defining the adaptive metabolism and general physiology of an organism. This thesis aims to discuss basic aspects of energy expenditure and their interactions with energy stores and body composition. In Paper I, we apply a new technique – high-resolution laser-Doppler imaging – to describe physiological regulatory features of adrenergically-stimulated blood flow in brown adipose tissue, and evaluate the validity of blood flow as a parameter to estimate nonshivering thermogenesis. Paper II focuses on the central regulation of body temperature. In the absence of bombesin receptor subtype-3, mice present an altered neurological body temperature setpoint, while peripheral thermogenic capacity remains intact. We conclude that brown adipose tissue malfunction is not the cause of the hypothermia observed in this mouse model. Paper III incorporates measurements of body temperature to the energy expenditure of different sources: basal metabolic rate, physical activity, thermic effect of food, and cold-induced thermogenesis. We describe basic aspects of dynamic insulation, energetic costs of circadian variation and hypothesize that physical activity may change the body temperature setpoint. Paper IV describes methodological issues related to glucose tolerance tests in obese mice. We conclude that the erroneous scaling of doses may affect the interpretation of metabolic health in mouse models, and suggest a new methodology. Paper V describes the outcomes caused by the expression of the human Cidea protein in adipose tissue of mice and suggests that this protein may clarify the link between adipose tissue expansion and healthy obesity. Paper VI explores the dissociation between thiazolidinedione-induced adipose tissue “browning” and reduced blood glycaemia. We demonstrate that although this pharmacological class tends to induce some level of brown adipose tissue recruitment, this phenomenon does not define its antidiabetic effects.

Place, publisher, year, edition, pages
Stockholm: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 2015. 55 p.
National Category
Physiology
Research subject
Physiology
Identifiers
urn:nbn:se:su:diva-115874 (URN)978-91-7649-156-0 (ISBN)
Public defence
2015-05-13, Nordenskiöldsalen, Geovetenskapens hus, Svante Arrhenius väg 12, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 4: Manuscript. Paper 5: Manuscript. Paper 6: Manuscript.

Available from: 2015-04-20 Created: 2015-04-06 Last updated: 2017-03-28Bibliographically approved

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