On adequate procedures for glucose tolerance tests in obese animals: Measurement of glucose tolerance in obesity
(English)Manuscript (preprint) (Other academic)
Routine procedures for glucose tolerance test in rodents utilize an amount of injected glucose that is proportional to total body weight (normally 2 mg per g body weight). Obese mice consist of much more chemically inert lipid than lean mice but have only marginal increases in lean body mass (the only compartment where glucose is distributed). Present procedures thus inevitably lead to a diagnosis of impaired glucose tolerance and enhanced insulin levels in obesity. Routine procedures should use fixed glucose amounts per lean body mass (or per mouse).
Research subject Physiology
IdentifiersURN: urn:nbn:se:su:diva-115871OAI: oai:DiVA.org:su-115871DiVA: diva2:800514